Abstract: There are provided herein, inter alia, complexes, compositions and methods for a vaccine for COVID-19. The cell-penetrating complexes provided herein may include a nucleic acid non-covalently bound to a cationic amphipathic polymer, the cationic amphipathic polymer including a pH-sensitive immolation domain. The cell-penetrating complexes provided herein are, inter alia, useful in vaccines, wherein the vaccine includes a ribonucleic acid including a sequence encoding a viral protein, a nucleic acid adjuvant and a cationic amphipathic polymer provided herein including embodiments thereof.

Abstract: There are provided herein, inter alia, cationic amphipathic polymers, complexes, and compositions comprising same, and methods for their use including for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acids, into a cell. The complexes, compositions and methods may facilitate delivery and targeted release of the therapeutic, diagnostic and imaging agents to particular cell types and tissues.

Abstract: A lined pipe joint with an improved collar seal and pipe lining end therefor. The pipe lining end is disposed at the end of the liner in the pipe. The lining end is adapted to shoulder against grouting material which holds the liner in place such that longitudinal contraction of the liner is prevented. The lining end also has a tapered bore therein which compensates for any inward deflection of the pipe section when threadingly engaged with a pipe fitting. The collar seal fits within the end of a pipe section which is to be connected to a pipe fitting. A sealing check valve on the collar seal provides sealing between the collar seal and the pipe section when pressure within the pipe section is greater than pressure outside the collar seal. The sealing check valve allows relief of pressure outside the collar seal into the pipe section when the pipe section is depressurized, so that damaging inward deflection of the pipe liner is prevented. A method of assembling the pipe joint is also disclosed.