Abstract: The field of biomarker detection and drug development is constantly in pursuit of more accurate, reliable, rapid, and inexpensive technology. Methods and systems for quantifying an analyte of interest disclosed herein include steps of obtaining a sample, spiking the sample with the analyte of interest, separating and detecting the sample using LC-MS, and calculating a linear regression of a sample using analytical techniques are described herein. Further, these methods and systems preclude the use and/or calculation of endogenous amounts of analyte present in a sample, yielding to methods and systems that provide increased speed and accuracy for measuring sample analytes.

Abstract: Disclosed herein are embodiments of methods for oligonucleotide analysis using a novel solid-phase extraction and hydrophilic-interaction liquid chromatography. The unique polar-based retention methods provided herein provide a high-recovery extraction. The methods improve assay reliability and reproducibility and reach picomolar sensitivity with the demonstrably beneficial accurate mass platform. Also disclosed herein are systems and computer program products for performing these methods.

Abstract: Methods of LC-MS analysis of oligonucleotides free of ion-pairing reagents are disclosed herein. Historically, ion-pairing reagents have been used for acceptable extraction and chromatography prior to mass spectral analysis. The disclosure herein presents methods free of ion-pairing reagents at each stage, from extraction through the LC-MS endpoint. Also disclosed herein are systems for performing these methods.

Abstract: Provided are a system and method of generating an aggregated stability map of one or more rotational sources associated with a heart rhythm disorder. In accordance therewith, a plurality of rotational area profile maps is accessed for a plurality of analysis intervals. Each of the profile maps includes rotation intensity values for a plurality of locations associated with rotation of the one or more rotational sources. An aggregated stability map is generated based on the profile maps, wherein the stability map includes a plurality of locations. Each location includes a rotation intensity value based at least on a filter number of highest rotation intensity values from corresponding locations of the profile maps, the filter number being automatically determined from a plurality of filter numbers such that the plurality of profile maps as filtered includes a predetermined number of rotation intensity values in excess of a threshold intensity value.

Abstract: Methods of LC-MS analysis of oligonucleotides free of ion-pairing reagents are disclosed herein. Historically, ion-pairing reagents have been used for acceptable extraction and chromatography prior to mass spectral analysis. The disclosure herein presents methods free of ion-pairing reagents at each stage, from extraction through the LC-MS endpoint. The methods improve assay reliability and reproducibility, extend column lifetimes, and decrease instrumental downtime previously necessary to remove ion-pairing reside. Also disclosed herein are systems for performing these methods.

Abstract: Provided are a system and method of generating an aggregated stability map of one or more rotational sources associated with a heart rhythm disorder. In accordance therewith, a plurality of rotational area profile maps is accessed for a plurality of analysis intervals. Each of the profile maps includes rotation intensity values for a plurality of locations associated with rotation of the one or more rotational sources. An aggregated stability map is generated based on the profile maps, wherein the stability map includes a plurality of locations. Each location includes a rotation intensity value based at least on a filter number of highest rotation intensity values from corresponding locations of the profile maps, the filter number being automatically determined from a plurality of filter numbers such that the plurality of profile maps as filtered includes a predetermined number of rotation intensity values in excess of a threshold intensity value.

Abstract: Provided is a system and method of generating an aggregated stability map of one or more rotational sources associated with a heart rhythm disorder. In accordance therewith, a plurality of rotational area profile maps is accessed for a plurality of analysis intervals. Each of the rotational area profile maps includes rotation intensity values for a plurality of locations associated with rotation of the one or more rotational sources. Thereafter, an aggregated stability map is generated map based on the plurality of rotational area profile maps, wherein the aggregated stability map includes a plurality of locations. Each location includes a rotation intensity value based at least on a filter level of highest rotation intensity values for that location from corresponding locations of the plurality of rotational area profile maps.

Abstract: The present technology regards a de-coupled V-hull structure for use with an armored vehicle, and energy absorbing crush elements suitable for mounting the V-hull structure in a de-coupled manner to the vehicle. The energy absorbing V-hull structure includes a sloped armor structure forming a cavity having a v-shaped cross-section and a plurality of reinforcing elements, including a backbone, hull stiffeners and lateral supports. The elements are coupled together and supported by energy absorber mounts, extending along each side of the structure. Crush elements suitable for decoupling the V-hull structure are also disclosed, having a uniquely designed housing, a plurality of plates positioned within the housing, and affixation means for securing the crush element to the underside of the vehicle and to the top of the V-hull structure.

Abstract: A system to generate a representation of a rhythm disorder that includes identifying remote or polar sources associated with a cardiac rhythm disorder is disclosed. The system includes generating a representation based on the cardiac information signals received from the sensors by transformation of spline-sensor locations of the catheter to x-y coordinate pairs of locations. A first offset is determined resulting from a perturbation to corresponding x-y coordinate pairs of locations associated with the representation, the first offset displacing coordinate pairs of sensor locations of the representation at least one unit of displacement in a first direction. A remote source associated with a cardiac rhythm disorder is identified when activations associated with the cardiac information signals rotate in sequence at least once, or emanate centrifugally for at least a first time period, the source being identified based on the representation as displaced.

Abstract: The disclosed technology regards a de-coupled V-hull structure for use with an armored vehicle, and energy absorbing crush elements suitable for mounting the V-hull structure in a de-coupled manner to the vehicle. The energy absorbing V-hull structure includes a sloped armor structure forming a cavity having a v-shaped cross-section and a plurality of reinforcing elements, including a backbone, hull stiffeners and lateral supports. The elements are coupled together and supported by energy absorber mounts, extending along each side of the structure. Crush elements suitable for decoupling the V-hull structure are also disclosed, having a uniquely designed housing, a plurality of plates positioned within the housing, and affixation means for securing the crush element to the underside of the vehicle and to the top of the V-hull structure.

Abstract: An example system and method of defining a rotational source associated with a heart rhythm disorder are disclosed. A plurality of center locations of wave fronts are calculated at a plurality of time points associated with the heart rhythm disorder. A rotational path that connects the plurality of center locations is then determined. Thereafter, a clinical representation that identifies a region of heart tissue associated with the rotational path is generated. The system and method can also determine a likely core associated with the rotational path. A plurality of relative diffusion shapes associated with the plurality of the center locations is calculated. A plurality of intersecting points of a smallest relative diffusion shape and other relative diffusion shapes is determined within the rotational path. A bounded polygon of the intersecting points is defined as the likely core.

Abstract: The disclosed technology regards a de-coupled V-hull structure for use with an armored vehicle, and energy absorbing crush elements suitable for mounting the V-hull structure in a de-coupled manner to the vehicle. The energy absorbing V-hull structure includes a sloped armor structure forming a cavity having a v-shaped cross-section and a plurality of reinforcing elements, including a backbone, hull stiffeners and lateral supports. The elements are coupled together and supported by energy absorber mounts, extending along each side of the structure. Crush elements suitable for decoupling the V-hull structure are also disclosed, having a uniquely designed housing, a plurality of plates positioned within the housing, and affixation means for securing the crush element to the underside of the vehicle and to the top of the V-hull structure.

Abstract: A system to generate a representation of a rhythm disorder that includes identifying remote or polar sources associated with a cardiac rhythm disorder is disclosed. The system includes generated a representation based on the cardiac information signals received from the sensors by transformation of spline-sensor locations of the catheter to x-y coordinate pairs of locations. A first offset is determined resulting from a perturbation to corresponding x-y coordinate pairs of locations associated with the representation, the first offset displacing coordinate pairs of sensor locations of the representation at least one unit of displacement in a first direction. A remote source associated with a cardiac rhythm disorder is identified when activations associated with the cardiac information signals rotate in sequence at least once, or emanate centrifugally for at least a first time period, the source being identified based on the representation as displaced.

Abstract: The disclosed technology regards a de-coupled V-hull structure for use with an armored vehicle, and energy absorbing crush elements suitable for mounting the V-hull structure in a de-coupled manner to the vehicle. The energy absorbing V-hull structure includes a sloped armor structure forming a cavity having a v-shaped cross-section and a plurality of reinforcing elements, including a backbone, hull stiffeners and lateral supports. The elements are coupled together and supported by energy absorber mounts, extending along each side of the structure. Crush elements suitable for decoupling the V-hull structure are also disclosed, having a uniquely designed housing, a plurality of plates positioned within the housing, and affixation means for securing the crush element to the underside of the vehicle and to the top of the V-hull structure.

Abstract: Provided is a system and method of generating an aggregated stability map of one or more rotational sources associated with a heart rhythm disorder. In accordance therewith, a plurality of rotational area profile maps is accessed for a plurality of analysis intervals. Each of the rotational area profile maps includes rotation intensity values for a plurality of locations associated with rotation of the one or more rotational sources. Thereafter, an aggregated stability map is generated map based on the plurality of rotational area profile maps, wherein the aggregated stability map includes a plurality of locations. Each location includes a rotation intensity value based at least on a filter level of highest rotation intensity values for that location from corresponding locations of the plurality of rotational area profile maps.

Abstract: An example system and method of defining a rotational source associated with a heart rhythm disorder are disclosed. A plurality of center locations of wave fronts are calculated at a plurality of time points associated with the heart rhythm disorder. A rotational path that connects the plurality of center locations is then determined. Thereafter, a clinical representation that identifies a region of heart tissue associated with the rotational path is generated. The system and method can also determine a likely core associated with the rotational path. A plurality of relative diffusion shapes associated with the plurality of the center locations is calculated. A plurality of intersecting points of a smallest relative diffusion shape and other relative diffusion shapes is determined within the rotational path. A bounded polygon of the intersecting points is defined as the likely core.

Abstract: An example system and method of determining a likely core of a rotational source associated with a heart rhythm disorder are disclosed. A plurality of relative diffusion shapes associated with wave fronts is calculated at a plurality of time points associated with the rotational source. The wave fronts associated with heart signals. A plurality of intersecting points of a smallest relative diffusion shape and other relative diffusion shapes is determined. A bounded polygon of the intersecting points is defined as the likely core.

Abstract: An example system and method of determining a likely core of a rotational source associated with a heart rhythm disorder are disclosed. A plurality of relative diffusion shapes associated with wave fronts is calculated at a plurality of time points associated with the rotational source. The wave fronts associated with heart signals. A plurality of intersecting points of a smallest relative diffusion shape and other relative diffusion shapes is determined. A bounded polygon of the intersecting points is defined as the likely core.

Abstract: An example system and method of defining a rotational source associated with a heart rhythm disorder are disclosed. A plurality of center locations of wave fronts are calculated at a plurality of time points associated with the heart rhythm disorder. A rotational path that connects the plurality of center locations is then determined. The system and method can also determine a likely core associated with the rotational path. A plurality of relative diffusion shapes associated with the plurality of the center locations is calculated. A plurality of intersecting points of a smallest relative diffusion shape and other relative diffusion shapes is determined within the rotational path. A bounded polygon of the intersecting points is defined as the likely core.