Patents by Inventor Robert Mierendorf

Robert Mierendorf has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Continuous Isocratic Affinity Chromatography
    Publication number: 20080053901
    Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.
    Type: Application
    Filed: August 30, 2007
    Publication date: March 6, 2008
    Applicant: SEMBA, INC.
    Inventors: Robert Mierendorf, Herbert Paaren, Anthony Grabski, Andrew Wilke, Joerg Baier, Anil Oroskar
  • Valve Module And Methods For Simulated Moving Bed Chromatography
    Publication number: 20080053543
    Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.
    Type: Application
    Filed: August 30, 2007
    Publication date: March 6, 2008
    Applicant: SEMBA, INC.
    Inventors: Joerg Baier, Robert Mierendorf, Anthony Grabski, Andrew Wilke, Anil Oroskar
  • Method for in vitro protein synthesis
    Patent number: 5998163
    Abstract: A method for performing coupled in vitro transcription and translation reactions is disclosed. In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
    Type: Grant
    Filed: January 13, 1999
    Date of Patent: December 7, 1999
    Assignee: Novagen, Inc.
    Inventors: Robert Mierendorf, Thomas R. Van Oosbree
  • Method for in vitro protein synthesis
    Patent number: 5895753
    Abstract: A method for performing coupled in vitro transcription and translation reactions is disclosed In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
    Type: Grant
    Filed: September 30, 1997
    Date of Patent: April 20, 1999
    Assignee: Novagen, Inc.
    Inventors: Robert Mierendorf, Thomas R. Van Oosbree
  • Protein ligand binding region mapping system
    Patent number: 5464745
    Abstract: A method for determining the nucleic acid sequence that encodes ligand binding domain on any protein encoded by a known gene includes the steps of non-specifically cleaving the coding region of the known gene, size-selecting the cleavage products, cloning the size-selected gene products into an expression vector active in a heterologous host, using a novel cloning strategy expressing the peptides produced by the clones and screening for clones that produce peptides that interact with an antibody or ligand of interest.
    Type: Grant
    Filed: March 31, 1993
    Date of Patent: November 7, 1995
    Assignee: Novagen, Inc.
    Inventors: Robert Mierendorf, Richard Garber, Robert Novy, Beth Hammer