Abstract: The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilises recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell tissue, organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto are also provided.

Abstract: A method of inducing, promoting or otherwise facilitating a change in the phenotype of an animal cell or group of animal cells including an animal comprising said cells. The modulation of phenotypic expression is conveniently accomplished via genotypic manipulation through such means as reducing translation of transcript to proteinaceous product. The ability to induce, promote or otherwise facilitate the silencing of expressible genetic sequences provides a means for modulating the phenotype in, for example, the medical, veterinary and the animal husbandry industries. Expressible genetic sequences contemplated by the present invention include not only genes normally resident in a particular animal cell (i.e., indigenous genes) but also genes introduced through recombinant means or through infection by pathogenic agents such as viruses.

Abstract: The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

Abstract: The present invention relates generally to a method for the detection of genetic expression in cells. More particularly, the present invention is directed to a method of monitoring the transcriptional activity of genetic elements including genes in a cell and more particularly to a method of determining at a quantitative, semi-quantitative or qualitative level the transcriptional activity of selected genetic elements in a cell. The present invention is further directed to a method for analyzing run-on transcription in cells and cellular organelles such as a nuclei, mitochondria and/or chloroplasts. The present invention further contemplates the use of real-time detection analysis in an amplification assay for the determination of run-on transcription in a cell and/or cellular organelles such as a nuclei, mitochondria and/or chloroplasts.

Abstract: The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.