Abstract: According to one embodiment, a data sources interface of a mainframe receives a transaction request from a user terminal communicating through a network. The transaction request is formatted in a web-based format. A data sources function is called to instruct a mainframe application to perform the requested transaction, and the instruction to the mainframe is formatted in the native format of the mainframe. A transaction result formatted in the native format of the mainframe is received from the mainframe application. The transaction result is modified according to the web-based format to yield a data source, and the data source is sent to the user terminal.

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3? terminus of one end and has a single stranded 5? overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5? overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences.

Abstract: According to one embodiment, a data sources interface of a mainframe receives a transaction request from a user terminal communicating through a network. The transaction request is formatted in a web-based format. A data sources function is called to instruct a mainframe application to perform the requested transaction, and the instruction to the mainframe is formatted in the native format of the mainframe. A transaction result formatted in the native format of the mainframe is received from the mainframe application. The transaction result is modified according to the web-based format to yield a data source, and the data source is sent to the user terminal.

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3? terminus of one end and has a single stranded 5? overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5? overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract: A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3? terminus of one end and has a single stranded 5? overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5? overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract: The present invention relates to cells, methods, compositions and kits for determining the concentration of virus in a stock, i.e., determining the titer of a viral stock.

Abstract: A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the topoisomerase to both termini of at least one end of a second ds nucleotide sequence is provided. Also provided is a method for generating a ds recombinant nucleic acid molecule covalently linked in one strand, by contacting two or more ds nucleotide sequences with a type IA topoisomerase under conditions such that one strand, but not both strands, of one or both ends of a first ds nucleotide sequence are covalently linked by the topoisomerase. Compositions for performing such methods, and compositions generated from such methods also are provided, as are kits containing components useful for conveniently practicing the methods.

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3? terminus of one end and has a single stranded 5? overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5? overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract: The present invention provides compositions and methods for the construction of nucleic acids comprising all or portion of a viral genome. Nucleic acid molecules of the invention may be constructed to contain multiple recombination and/or topoisomerase recognition sites. The compositions include vectors having multiple recombination sites with unique specificity that contain all or a portion of a viral genome. The methods permit the insertion of a sequence of interest into a viral genome using recombinational and/or topoisomerase-mediated cloning. The present invention also provides methods of constructing recombinant virus, methods of expressing polypeptides, and methods of expressing fusion polypeptides.

Abstract: In general, the present invention provides compositions for the cellular delivery of nucleic acids, polypeptides and/or flourophores, molecular complexes comprising fluorescent molecules or moieties, nucleic acids and polypeptides, and methods of making and using such compositions. Light-activated dispersal of the complexes leads to the intracellular release of one or more nucleic acids and/or peptides from the compositions or complexes. The biological activities of nucleic acids, polypeptides and flourophores may be repressed within the complexes, and these activities are restored upon release from the complexes.

Abstract: The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that contains the target gene) attached to a translocating polypeptide. In another embodiment, expression of a target gene product in a cell in culture that contains a target gene under control of one or more regulatory elements is modulated by contacting the cell in culture with one or more regulatory agents attached to a translocating polypeptide. The one or more regulatory agents are translocated into the cell in culture and interact therein with the one or more regulatory elements to modulate expression of the target gene product by the cell.

Abstract: The present invention provides compositions and methods for producing fusion proteins that comprise an amino acid sequence tag. The amino acid sequence tag may be an amino acid sequence that is capable of being post-translationally modified; for example, the amino acid sequence may be an amino acid sequence that is capable of being biotinylated. The amino acid sequence tag may also be an amino acid sequence that is recognized by an antibody (or fragment thereof) or other specific interacting reagent. The invention includes isolated nucleic acid molecules comprising one or more nucleic acid sequences which encode an amino acid sequence tag. The nucleic acid molecules of the invention may also comprise one or more recombination sites and/or one or more topoisomerase recognition sites and/or one or more topoisomerases.

Abstract: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first ds nucleic acid molecule, which has a topoisomerase linked to a 3′ terminus of one end and has a single stranded 5′ overhang at the same end, with a second ds nucleic acid molecule having a blunt end, such that the 5′ overhang can hybridize to a complementary sequence of the blunt end of the second nucleic acid molecule, and the topoisomerase can covalently link the ds nucleic acid molecules. The methods are simpler and more efficient than previous methods for covalently linking nucleic acid sequences, and the compositions and kits facilitate practicing the methods, including methods of directionally linking two or more ds nucleic acid molecules.

Abstract: The present invention provides a method and composition for on-line treatment of furnace walls that are subject to deposition of highly reflective ash. The method involves exposing the walls to a darkening agent, or a combination of a darkening agent and a fluxing agent. The darkening agent, or the combined darkening agent and fluxing agent, may be mixed with the coal before it enters the boiler. Preferably, the appropriate walls of the boiler are treated directly with the darkening agent. A preferred embodiment involves direct application of the darkening agent to the water wall.

Abstract: A method and composition for control of in-transit and residual dust from coal or coke is provided. The method involves spraying the coal or coke with fluid, preferably water, containing an additive comprised of approximately 90-99% by weight of lignosulfonate and 1-10% by weight of a polyacrylate, a polyvinylalcohol, or a polyacrylamide. Sufficient fluid should be used to coat the coal or coke. The amount of additive necessary to prevent dusting will vary according to the conditions to which the coke or coal will be subjected.

Abstract: A method and composition for control of in-transit and residual dust, erosion, and wetting of coal or coke feeds is provided. The method involves spraying the coal or coke with a solution comprising a fluid, preferably water, containing a sufficient amount of an additive comprising (a) approximately 90-99 wt % of a binder, preferably a lignosulfonate, a polyether, a polyol, or a byproduct produced during the refining of a natural sugar, and (b) 1-10 wt % of a polyacrylate, a polyvinylalcohol, or a polyacrylamide. The additive also preferably contains a surfactant. The amount of solution/additive that is necessary will vary according to the desired result and the conditions to which the coke or coal will be subjected.

Abstract: A method and composition for control of in-transit and residual dust from coal or coke is provided. The method involves spraying the coal or coke with fluid, preferably water, containing an additive comprised of approximately 90-99% by weight of lignosulfonate and 1-10% by weight of polyacrylic acid. Sufficient fluid should be used to coat the coal or coke. The amount of additive necessary to prevent dusting will vary according to the conditions to which the coke or coal will be subjected.

Abstract: A microwave oven and a refrigerator are combined in a single cabinet and share line power supplied to the cabinet. Door interlocks and a manually settable time are provided for disconnecting electrical supply to the compressor of the refrigerator during operation of the magnetron of the microwave oven. The circuitry provided ensures that electrical supply is reconnected to the compressor upon expiration of the timer setting, even if the door of the oven is incidentally left open after use.

Abstract: A microwave oven and a refrigerator are combined into a single cabinet housing a shared power supply with an electrical supply disconnect for the magnetron of the oven during a start-up power demand by the compressor of the refrigerator and for a period of time depending upon a rating of the compressor.