Patents by Inventor Robert P. St. Onge

Robert P. St. Onge has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multiplex production and barcoding of genetically engineered cells
    Patent number: 12416015
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: September 16, 2025
    Assignees: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, BRANDEIS UNIVERSITY
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber
  • Scalable method for isolation and sequence-verification of oligonucleotides from complex libraries
    Patent number: 11505794
    Abstract: A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.
    Type: Grant
    Filed: August 19, 2020
    Date of Patent: November 22, 2022
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Robert P. St. Onge, Ulrich Schlecht, Sasha F. Levy, Ronald W. Davis, Joseph Horecka
  • SCALABLE METHOD FOR ISOLATION AND SEQUENCE-VERIFICATION OF OLIGONUCLEOTIDES FROM COMPLEX LIBRARIES
    Publication number: 20210246440
    Abstract: A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.
    Type: Application
    Filed: August 19, 2020
    Publication date: August 12, 2021
    Inventors: Robert P. St. Onge, Ulrich Schlecht, Sasha F. Levy, Ronald W. Davis, Joseph Horecka
  • Scalable method for isolation and sequence-verification of oligonucleotides from complex libraries
    Patent number: 10787661
    Abstract: A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: September 29, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Robert P. St. Onge, Ulrich Schlecht, Sasha F. Levy, Ronald W. Davis, Joseph Horecka
  • MULTIPLEX PRODUCTION AND BARCODING OF GENETICALLY ENGINEERED CELLS
    Publication number: 20200270632
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Application
    Filed: September 14, 2018
    Publication date: August 27, 2020
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber
  • SCALABLE METHOD FOR ISOLATION AND SEQUENCE-VERIFICATION OF OLIGONUCLEOTIDES FROM COMPLEX LIBRARIES
    Publication number: 20160122748
    Abstract: A novel method for preparing sequence-verified oligonucleotides is disclosed. In particular, the invention relates to a simple, affordable, and scalable method that combines high-throughput mating of yeast clones, a unique selectable system for combining DNA sequences in yeast, and next-generation sequencing. This method allows sequence-verified oligonucleotides to be readily isolated from complex libraries.
    Type: Application
    Filed: October 30, 2015
    Publication date: May 5, 2016
    Inventors: Robert P. St. Onge, Ulrich Schlecht, Sasha F. Levy, Ronald W. Davis, Joseph Horecka