Patents by Inventor Robert S. Plumb
Robert S. Plumb has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200096525Abstract: The present disclosure relates to methodologies, systems, and devices for screening lipids. The technique includes selecting a set of standards to identify at least one desired class of lipids, spiking the standards into a biological sample to form a sample matrix, extracting the lipids from the sample matrix, and introducing the sample matrix into a chromatography system to separate the desired class of lipids. Once the lipids are separated into different lipid classes using HILIC chromatography, they are directed to a detector, and the separated lipids are quantified based on a comparison between the measured detector response and a calibration curve generated with a known concentration of the selected set of standards.Type: ApplicationFiled: September 19, 2019Publication date: March 26, 2020Applicant: Waters Technologies CorporationInventors: Lee Gethings, Giorgis M. Isaac, Nyasha Clarence Munjoma, Robert S. Plumb, Paul Rainville
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Patent number: 10024826Abstract: An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.Type: GrantFiled: August 22, 2012Date of Patent: July 17, 2018Assignee: WATERS TECHNOLOGIES CORPORATIONInventors: James P. Murphy, Michael J. Tomany, Joseph D. Michienzi, Paul Rainville, Robert S. Plumb, Geoff C. Gerhardt, Moon Chul Jung
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Publication number: 20180094293Abstract: Provided herein are methodologies where a glycosylated protein or peptide is subjected to peptide bond cleavage to produce a glycan amino acid complex wherein the N-linked or O-linked glycan is attached. A derivatization reagent is then attached to the N terminus of the amino acid to provide a labeled glycan amino acid complex. The labeled glycan amino acid complex is then separated from the matrix via one or more methods including HILIC SPE, and injected directly onto an LC or LC/MS system for analysis, detection and characterization of the glycosylated protein or the peptide.Type: ApplicationFiled: September 29, 2017Publication date: April 5, 2018Applicant: Waters Technologies CorporationInventors: Paul Rainville, Matthew A. Lauber, Lee Gethings, Robert S. Plumb, Eoin Cosgrave, Darryl W. Brousmiche
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Patent number: 9390897Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: GrantFiled: September 22, 2014Date of Patent: July 12, 2016Assignee: Micromass UK LimitedInventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Publication number: 20150008319Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: ApplicationFiled: September 22, 2014Publication date: January 8, 2015Inventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Patent number: 8841606Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: GrantFiled: October 5, 2011Date of Patent: September 23, 2014Assignee: Micromass UK LimitedInventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Publication number: 20140208836Abstract: An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.Type: ApplicationFiled: August 22, 2012Publication date: July 31, 2014Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: James P. Murphy, Michael J. Tomany, Joseph D. Michienzi, Paul Rainville, Robert S. Plumb, Geoff C. Gerhardt, Moon Chul Jung
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Publication number: 20120112055Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: ApplicationFiled: October 5, 2011Publication date: May 10, 2012Applicant: MICROMASS UK LIMITEDInventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Publication number: 20120052522Abstract: Embodiments of the present invention feature methods and apparatus in which fluids circulating between a plurality of vessels containing different cell types or tissues are monitored for metabolites following the introduction of a sample.Type: ApplicationFiled: November 17, 2009Publication date: March 1, 2012Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: Jose Castro-Perez, Robert S. Plumb, Jennifer Harnisch Granger
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Patent number: 8063357Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: GrantFiled: July 1, 2005Date of Patent: November 22, 2011Assignee: Micromass UK LimitedInventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Patent number: 8017908Abstract: Chromatograms and mass spectra produced by an LC/MS system are analyzed by creating a two-dimensional data matrix of the spectral and chromatographic data. The two-dimensional matrix can be created by placing the spectra generated by the mass spectrometer portion of the LC/MS system in successive columns of the data matrix. In this way, the rows of the data matrix correspond to chromatographic data and the columns of the data matrix correspond to the spectra. A two-dimensional filter is specified and applied to the data matrix to enhance the ability of the system to detect peaks associated with ions. The two-dimensional filter is specified according to desired criteria. Rank-1 and rank-2 filters can be specified to improve computational efficiency. One method of applying the two-dimensional filter is through convolution of the data matrix with the two-dimensional filter to produce an output data matrix. Peaks corresponding to detected ions are identified in the output data matrix.Type: GrantFiled: December 18, 2009Date of Patent: September 13, 2011Assignee: Waters Technologies CorporationInventors: Marc V. Gorenstein, Robert S. Plumb, Chris L. Stumpf
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Patent number: 7938961Abstract: Disclosed herein are an apparatus and method for effectuating increased chromatographic efficiency in a capillary column by employing a retaining frit disposed within an analytical capillary. By disposing the retaining frit within the analytic capillary, the void volume is significantly minimized. The columns and methods described herein produce a simplified analytical capillary and retaining frit apparatus that provides greater chromatographic efficiency. Additionally, the column of the instant invention maintains chromatographic fidelity by reducing the transfer diameter as well as facilitating fluidic connections in situ.Type: GrantFiled: August 2, 2005Date of Patent: May 10, 2011Assignee: Waters Technologies CorporationInventors: Robert S. Plumb, Chris L. Stumpf, Jennifer H. Granger
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Publication number: 20110095176Abstract: A method of searching for potentially unknown metabolites of pharmaceutical compounds is disclosed. The accurate mass of a pharmaceutical compound will generally be known and can be rendered in the form of an integer nominal mass or mass to charge ratio component and a decimal mass or mass to charge ratio component. Possible metabolites are searched for on the basis of having a decimal mass or mass to charge ratio component which is substantially very similar to the decimal mass or mass to charge ratio of the parent pharmaceutical compound.Type: ApplicationFiled: July 1, 2005Publication date: April 28, 2011Applicant: MICROMASS UK LIMITEDInventors: Jose M. Castro-Perez, Alan Millar, Robert S. Plumb
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Publication number: 20100187414Abstract: Chromatograms and mass spectra produced by an LC/MS system are analyzed by creating a two-dimensional data matrix of the spectral and chromatographic data. The two-dimensional matrix can be created by placing the spectra generated by the mass spectrometer portion of the LC/MS system in successive columns of the data matrix. In this way, the rows of the data matrix correspond to chromatographic data and the columns of the data matrix correspond to the spectra. A two-dimensional filter is specified and applied to the data matrix to enhance the ability of the system to detect peaks associated with ions. The two-dimensional filter is specified according to desired criteria. Rank-1 and rank-2 filters can be specified to improve computational efficiency. One method of applying the two-dimensional filter is through convolution of the data matrix with the two-dimensional filter to produce an output data matrix. Peaks corresponding to detected ions are identified in the output data matrix.Type: ApplicationFiled: December 18, 2009Publication date: July 29, 2010Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: Marc V. Gorenstein, Robert S. Plumb, Chris L. Stumpf
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Patent number: 7645984Abstract: Chromatograms and mass spectra produced by an LC/MS system are analyzed by creating a two-dimensional data matrix of the spectral and chromatographic data. The two-dimensional matrix can be created by placing the spectra generated by the mass spectrometer portion of the LC/MS system in successive columns of the data matrix. In this way, the rows of the data matrix correspond to chromatographic data and the columns of the data matrix correspond to the spectra. A two-dimensional filter is specified and applied to the data matrix to enhance the ability of the system to detect peaks associated with ions. The two-dimensional filter is specified according to desired criteria. Rank-1 and rank-2 filters can be specified to improve computational efficiency. One method of applying the two-dimensional filter is through convolution of the data matrix with the two-dimensional filter to produce an output data matrix. Peaks corresponding to detected ions are identified in the output data matrix.Type: GrantFiled: February 11, 2005Date of Patent: January 12, 2010Assignee: Waters Technologies CorporationInventors: Marc V. Gorenstein, Robert S. Plumb, Chris L. Stumpf