Abstract: A method of reducing regurgitation between native leaflets of an atrioventricular heart valve includes advancing a delivery catheter through a sheath, wherein the delivery catheter has a valve leaflet coaptation element mounted over a distal end portion. The coaptation element is positioned within the heart valve and is permitted to radially expand from a compressed configuration to an enlarged configuration for filling a gap between the native leaflets of the heart valve. After deployment, the position of the coaptation element is fixed relative to the heart valve, thereby reducing regurgitation between the native leaflets of the heart valve and improving heart function.

Abstract: A system comprise a server configured to communicate vehicle information with a vehicle transceiver of a vehicle moving along a vehicle route and communicate drone information with a drone transceiver of a drone moving along a drone route. A computing device with a memory and a processor may be configured to communicatively connect with the server, process the vehicle information and the drone information, identify a plurality of pickup locations based in part on the vehicle information and drone information, select at least one of the plurality of pickup locations based in part on a priority score associated with a travel time to or wait time for each of the plurality of pickup locations, and update the drone route based in part on the selected pickup location.

Abstract: A system comprise a server configured to communicate vehicle information with a vehicle transceiver of a vehicle moving along a vehicle route and communicate drone information with a drone transceiver of a drone moving along a drone route. A computing device with a memory and a processor may be configured to communicatively connect with the server, process the vehicle information and the drone information, identify a plurality of pickup locations based in part on the vehicle information and drone information, select at least one of the plurality of pickup locations based in part on a priority score associated with a travel time to or wait time for each of the plurality of pickup locations, and update the drone route based in part on the selected pickup location.

Abstract: Epidermal Growth Factor (EGF) is produced by recombinant DNA technology in Pichia pastoris yeast cells.

Abstract: The present invention pertains to a process for recovering hepatitis B surface antigen from recombinant Pichia pastoris cells comprising:(a) lysing said yeast cells in the presence of a buffered chaotropic salt and separating the hepatitis B surface antigen-containing supernatant from said lysed cells;(b) subjecting the heptatis B surface antigen-containing supernatant obtained in step (a) to conditions suitable to precipitate lipids and contaminant proteins from said supernatant;(c) subjecting the hepatitis B surface antigen-containing supernatant obtained in step (b) to diafiltration;(d) contacting the hepatitis B surface antigen-containing retentate obtained in step (c) with silica;(e) washing contaminant proteins from the resulting silica-adsorbed hepatitis B surface antigen with a buffer having a pH within the range of 6-8;(f) eluting the hepatitis B surface antigen from the silica with a buffered eluant having a pH within the range of 9.5-11.0 containing from 0.