Abstract: Reader and plate methods, operations, and systems for observing a biological sample are shown and described. In one embodiment, a device includes an imaging device and a variable tray holder nest adapted to perform colony counting to monitor biological growth, when present.

Abstract: Reader and plate methods, operations, and systems for observing a biological sample are shown and described. In one embodiment, a device for observing biological growth, when present, on a growth plate, includes an imaging device and a variable positioning nest. The nest may include a first sunken frame to receive a first growth plate, and an offset second sunken frame to receive a distinct second growth plate in an operating position offset about the first operating position.

Abstract: ABSTRACT: An instrument for processing droplets in a microfluidic system. The instrument captures a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The instrument also processes each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. This involves processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The instrument controls a microfluidic process performed on the droplet, e.g. droplet dispensing, responsive to the estimated number of cells or other entities in the droplet. The instrument uses the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

Abstract: Reader and plate methods, operations, and systems for observing a biological sample are shown and described. In one embodiment, a device for observing biological growth, when present, on a growth plate, includes an imaging device and a variable positioning nest. The nest may include a first sunken frame to receive a first growth plate, and an offset second sunken frame to receive a distinct second growth plate in an operating position offset about the first operating position.

Abstract: Example biodegradable tubular members and methods of producing biodegradable tubular members are described. A biodegradable product includes an elongated tubular member. The elongated tubular member includes one or more cellulose esters and a plurality of pores in the tubular member. The plurality of pores are sized and structured in the elongated tubular member to allow permeation or infiltration of at least one of water or bacteria into at least a portion of the plurality of pores and promote biodegradability of the elongated tubular member.

Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.

Abstract: In-line testing and product delivery assemblies, methods, operations, and systems are shown and described. In one embodiment, an in-line testing and product delivery system includes a supply of product having at least one outlet with a valve closure and a downstream delivery line, a recirculation closed loop in fluid communication with the outlet and supply, and a reader to generate a rapid test result from a single use assay for detection of a presence or an absence of an analyte in the supply. The result provides monitoring of a detection of the analyte to block release of product supply into the delivery line.

Abstract: A safety harness is provided for use on ski/chair-lifts for restraining a smaller rider. The safety harness comprises a body section having a rear panel, first and second girth panels, and first and second shoulder panels, the panels being configured generally in the form of a vest. The safety harness further comprises at least one strap for positioning and tightening the body section on the smaller rider. The rear panel is provided with a retaining loop, the retaining loop being sized to permit an adjacently positioned adult rider to place an arm therethrough, so as to restrain the smaller rider.

Abstract: A non-volatile memory cell comprises a first floating gate transistor having a source, a drain, and a gate electrically coupled to a row line. A second floating gate transistor has a source, a drain, and a gate electrically coupled to the row line. A first p-channel MOS transistor has a source, a drain, and a gate, the drain of the first p-channel MOS transistor electrically coupled to the drain of the first floating gate transistor forming a first common node. A second p-channel MOS transistor has a source, a drain, and a gate, the first drain of the second p-channel MOS transistor electrically coupled to the drain of the second floating gate transistor forming a second common node, the gate of the second p-channel MOS transistor electrically coupled to the first common node, and the second common node electrically coupled to the gate of the first p-channel MOS transistor.

Abstract: An aspect of the disclosure is a method and device for the detection of antibiotics in a sample. Embodiments include a microbial culture with growth indicators and sensitivity adjustment reagents. Adjustment reagents include a variety of antibiotic binders that can be added to microbial culture to reduce sensitivity to certain antibiotics. Some embodiments include multiple buffers with a variety of pKa ranges. Some embodiments also include a complete all-in-one apparatus for sampling and testing.

Abstract: Described herein is a method of controlling call admission for packet switched networks, each network including at least two local area networks (50, 60) and a connecting network (70). The method comprises determining success rates of previous calls from a first local area network to a second local area network and deciding to drop the call attempt based on the success rates of previous calls. In one embodiment, the current packet loss rate for calls from the first local area network to the second local area network is also determined, and the decision to drop the call attempt is based on that current packet loss rate. Additionally, the decision to drop the call attempt may be based on both the current packet loss rate and the success rates of previous calls.

Abstract: Described herein is a method of controlling calls for packet switched networks, each network including at least two local area networks (50, 60) and a connecting network (70). The method comprises the steps of determining an acceptable packet loss rate for a call to be established between two of the local area networks, comparing actual packet loss rate to the acceptable packet loss rate, and dropping the call if the actual packet loss rate is greater than the acceptable packet loss rate. determining for how long a period the actual packet loss rate has been happening and utilising that period in deciding to drop the call. A recorded announcement may be played when the call is to be dropped. Alternatively, the priority of the transmission of the continuous stream of data can be changed when the actual packet loss rate is not acceptable and the above steps are repeated. Ideally, data relating to dropped calls is stored for future use.

Abstract: Described herein is a method of controlling call admission for packet switched networks, each network including at least two local area networks (50, 60) and a connecting network (70). The method comprises transmitting a burst 5 of trial data of the same size as the packet to be transmitted from a first node (52, 54) in a first local area network (50) to a second node (62, 64) in a second local area network (60) via the connecting network (70). The connecting network (70) comprises a plurality of routing nodes (72, 74, 76, 78, 80) for routing the burst of trial data of the same size as the packet to be transmitted from a first node (52, 54) in a first local area network (50) to second node (62, 64) in a second local area network (60) via the connecting network (70). The connecting network (70) comprises a plurality of routing nodes (72, 74, 76, 78, 80) for routing the burst of trial data to the second node in the second local area network along a particular path.

Abstract: Raw material containing precious metals and heavy minerals is introduced into a comminuting chamber. The raw material falls onto rotating chains which drive the material against the side wall of the chamber with sufficient velocity to cause the raw material to fracture. Air flows upwardly at the side wall and classifies the particles into a first fraction which falls to the floor of the chamber and a second fraction which is carried upward to a trommel. Large particles from the trommel are recycled to the chamber while fines are discarded as tailings. The rate of upward flow of air in the chamber, the rate of rotation of the chains and the size of particles separated by the trommel are all adjusted in order to ensure that the particles that collect on the floor of the chamber are rich in precious metals and heavy minerals.

Abstract: There is disclosed amembrane assisted evaporation process (MAE) for economically and reliably removing water added to brine. The process comprises using low-grade waste heat and air to evaporate water from diluted salt brines when the water moves across a membrane in a liquid state.

Abstract: Raw material containing precious metals and heavy minerals is introduced into a comminuting chamber. The raw material falls onto rotating chains which drive the material against the side wall of the chamber with sufficient velocity to cause the raw material to fracture. Air flows upwardly at the side wall and classifies the particles into a first fraction which falls to the floor of the chamber and a second fraction which is carried upward to a trommel. Large particles from the trommel are recycled to the chamber while fines are discarded as tailings. The rate of upward flow of air in the chamber, the rate of rotation of the chains and the size of particles separated by the trommel are all adjusted in order to ensure that the particles that collect on the floor of the chamber are rich in precious metals and heavy minerals.

Abstract: There is disclosed a process for denitrifying tobacco materials and removing barium from tobacco materials, comprising mixing an aqueous-immiscible organic solvent containing a crown ether with an aqueous solution containing soluble components from tobacco materials, agitating this mixture, and separating the organic phase containing a crown ether-cation-nitrate (or nitrite) complex from the aqueous phase containing the denitrified tobacco materials, wherein the cation consists essentially of barium and potassium. There is further disclosed a process for eliminating tobacco-specific nitrosamines (TSNAs) from cured, denitrified tobacco material, comprising contacting the denitrified tobacco material with a trapping sink, wherein the trapping sink comprises a select transition metal complex which is readily nitrosated to form a nitrosyl complex with little kinetic or thermodynamic hindrance.