Abstract: The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.

Abstract: The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.

Abstract: The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.

Abstract: This disclosure provides a method comprising: a) clamping the top and bottom strands of a double stranded DNA molecule to produce a duplex in which the top and bottom strands are linked; b) denaturing the duplex to produce a denatured product; and c) renaturing the denatured product in the presence of a labeled oligonucleotide that is complementary to a sequence of nucleotides in the double stranded DNA molecule, thereby producing a D-loop-containing product. Kits for performing the method and products made by the method are also provided.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip. A flexible sleeve extends downwardly from the flange member. The toilet seal further includes a compressible member having an upper surface disposed adjacent to a lower surface of the flange member. The compressible member further has a lower surface and a plurality of ridges extend downwardly from the lower surface of the compressible member.

Abstract: The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.

Abstract: The present application provides for IL-2 muteins, compositions comprising the same, and methods of using the same.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: Methods for making a synthetic nucleic acid which comprise: (a) identifying a conflicting nucleotide sequence in a target sequence; (b) inserting a masking sequence into the conflicting sequence to produce a disrupted target sequence, wherein: (i) the masking sequence comprises recognition sites for one or more Type IIS restriction endonucleases; and (ii) digestion of said disrupted target sequence by said one or more Type IIS restriction endonucleases followed by re-ligation reconstitutes the target sequence; (c) synthesizing a polynucleotide comprising the disrupted target sequence using polymerase chain assembly; and (d) removing the masking sequence from said polynucleotide by digesting said polynucleotide with said one or more Type IIS restriction endonucleases followed by re-ligation of the digestion product, thereby producing a polynucleotide comprising said target sequence.

Abstract: Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together. Kits and compositions relating to the method are also provided.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: Certain exemplary embodiments can provide an apparatus wearable by a user. The apparatus can comprise a biometric sensor constructed to generate signals based upon measurements of the user. The apparatus can comprise a processor constructed to determine a significant detrimental change in the user via an algorithm based upon the signals.

Abstract: Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together. Kits and compositions relating to the method are also provided.

Abstract: A toilet seal for sealing between a plumbing fixture discharge and a waste drainpipe outlet is described. The toilet seal comprises a flange member having an inwardly extending flexible lip and one or more raised ridges defining an annular seal extending upwardly from an upper surface of the flange member. The toilet seal further includes a radiused wall extending downwardly from the flange member, which cooperates with the flange member to define a cavity for receiving a compressible member. The toilet seal further comprises a flexible sleeve extending downwardly from the radiused wall. Related methods are described.

Abstract: The present invention is a traveling dice game that includes a base casing, a pair of dice rolled to control a plurality of actions one or more players take while playing the traveling dice game, a pencil and a writing tablet that maintains a record of a plurality of notes or information related to the traveling dice game. The game also includes a plurality of pins, a top cover removably placed on the base casing to cover the base casing, a handle disposed on the base casing that is grasped to facilitate carrying the traveling dice game and a fastener disposed on the traveling dice game to releasably secure the top cover to the base casing. The pair of dice determines a number of plurality of pin combinations that are placed upward and downward that determine a number of points generated by each player.