Abstract: A system and method for identifying an unknown substance in a sample comprising multiple entities. A method may comprise generating a RGB image representative of a sample and assessing said RGB image to identify at least one region of interest. This region of interest may he assessed to generate a spatially accurate wavelength resolved image, which may be a hyperspectral image. This spatially accurate wavelength resolved image may comprise a fluorescence, Raman, near infrared, short wave infrared, mid wave infrared and/or long wave infrared image. This spatially accurate wavelength resolved image may be assessed to identify said unknown substance. A system may comprise: a reference database, a first detector for generating an RGB image, a second detector for generating a spatially accurate wavelength resolved image, and a means for assessing said RGB image and said spatially accurate wavelength resolved image.