Abstract: A tube sealing device for sealing a resilient thermoplastic tube. The tube sealing device includes a base that supports a resilient thermoplastic tube in a fixed position relative to the base. The heating tool and the cooling tool are coupled to the base for movement relative to each other. Relative movement between the heating tool and the base aligns the heating tool with a portion of the resilient thermoplastic tube. The heating tool operates to contact, pinch and heat the portion of the resilient thermoplastic tube. Relative movement between the cooling tool and the base aligns the cooling tool with the same portion of the resilient thermoplastic tube. The cooling tool operates to contact, pinch and cool the resilient thermoplastic tube to form a seal.