Abstract: Provided herein are compositions, methods, and kits for detection of Entamoeba nucleic acids. Some embodiments relate to detection of E. histolytica but not E. dispar. Some embodiments relate to quantification of levels of E. histolytica.

Abstract: A protein-fortified frozen dessert formulation and the process for making it are described. In particular, a sweet mix of 10-20% w/w sugar, 0-10% w/w flavouring, 0-20% w/w fat, 10-15% w/w milk solids, 0-11% w/w protein and 50-65% w/w water is prepared, preheated, pasteurized, homogenized and aged. The aged sweet-mix is then blended with yogurt and optionally more protein prior to freezing. Alternatively, a mix of 10-20% w/w sugar, 0-10% w/w flavouring, 0-20% w/w fat, 10-15% w/w milk solids, 5-15% w/w protein, 5-20% yogurt and 50-65% w/w water is prepared, preheated, pasteurized, homogenize and aged and frozen to a low target overrun.

Abstract: A cryogenic fluid jet is used in an apparatus and a method for remote cooling of a cutting tool engaged in machining a workpiece under high-energy conditions, such as high-speed machining, hard-turning, cutting of difficult to machine materials, and combinations thereof. The apparatus and method use a stabilized, free-expanding cryogenic fluid jet having a pulse cycle time less than or equal to about 10 seconds. The apparatus and method increase the cleanliness of machined parts and chips and machining productivity of hard but brittle tools, including but not limited to tools which should not be cooled with conventional cooling fluids.