Abstract: A 3D object (the “New Object”) is fabricated layer by layer by 3D printing. The shape and relative dimensions of the various parts of the New Object match that of another 3D object (the “Target Object”). In addition, the exterior of the New Object appears to be a photographic likeness of the Target Object. The “photographic” likeness is created by variations in visual characteristics of materials in the layers comprising the New Object, and in particular by variations at or near the surface of the New Object. Thus, the photographic likeness is an integral part of these layers comprising the New Object. An object is scanned, from which a texture map is obtained. A CAD model is sliced into slices (bit maps files) which are then colored by a program with the boundary to match the color or gray scale to color the appropriate pixels, derived from the texture map.

Abstract: Cement or other liquid-like material fills the hollow tubes of a machine tool under construction. The machine tool structures are held rigidly against a fixture while the substance dries. The machine tool so constructed is relatively lightweight and rigid, and obviates the need for precision machining of large portions of the apparatus.

Abstract: In an exemplary embodiment of this invention, corrugated board is folded into a building system. The building system comprises a plurality of blocks and connectors. The blocks are rectangular cuboid in shape. The bottom and top sides of the blocks have rectangular arrays of holes. The blocks may be releasably connected by aligning the top holes of one block with the bottom holes of another block, and then inserting connectors into the aligned pairs of holes, one connector per aligned paid of holes. The ends of the connectors that are inserted are circular or equiangular polygonal in shape. In some embodiments, compression is used to releasably hold the connectors in place.

Abstract: Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of polypeptides containing unnatural amino acids at one or more specified residues of the polypeptide.

Abstract: Bacterial flagellin protein is modified to improve adjuvant activity.

Abstract: Methods, microbial strains and reaction mixtures for cell-free synthesis of polypeptides are provided. The methods of the invention utilize a reaction mixture comprising microbial cell extracts that are modified in the protein component relative to an extract from a native cell. The modification may be one or both of (i) increased levels of proteins that increase synthetic yield; and (ii) decreased levels of proteins that decrease synthetic yield. The modification may result from a genetic modification of the microbial cell, or from ex vivo supplementation or depletion of an extract.

Abstract: Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane-associated polypeptides.

Abstract: Bacterial immunity proteins are utilized to increase immune response to an antigen of interest.

Abstract: In an illustrative implementation of this invention, a 3D object comprises substrate layers infiltrated by a hardened material. The 3D object is fabricated by a method comprising the following steps: Position powder on all or part of a substrate layer. Repeat this step for the remaining substrate layers. Transform the powder into a substance that flows and subsequently hardens into the hardened material. The hardened material solidifies in a spatial pattern that infiltrates positive regions in the substrate layers and does not infiltrate negative regions in the substrate layers.

Abstract: Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane-associated polypeptides.

Abstract: In an illustrative implementation of this invention, a mechanical computer is manufactured without a separate step of assembly. This is achieved by producing parts in a three dimensional pattern (in relation to each other) such that, when this manufacturing is completed, the components are already assembled to form a computer or part of a computer. The second step of assembling the parts into a desired three dimensional pattern is avoided, because the parts, when made, are already arranged in that 3D pattern.

Abstract: Biological macromolecules are synthesized in vitro under conditions and in a reaction composition wherein oxidative phosphorylation is activated and protein folding is improved.

Abstract: Compositions and methods are provided for the in vitro synthesis of biological molecules in reaction mixtures comprising anti-foam agents. The reaction mix comprising antifoam agent may be a scaled up reaction, e.g. in reaction volume greater than at least about 15 ?l. Reactions may be performed in various reactors, as known in the art, which include stirred reactors, bubble-column reactors; and the like.

Abstract: Compositions and methods are provided for the cell-free synthesis of active reprogramming factor polypeptides. The reprogramming factors may be synthesized as fusion proteins comprising a permeant domain, such as polyarginme. The cell free-synthesis may be conducted at about 25 C in a bacterial cell extract from genetically alterd cells having decreased endogenous protease activity Further, the proteins may comprise a fusion partner which enhances solubility and may be refolded on a column.

Abstract: A distributed heterogeneous or homogeneous file storage system divides logical responsibility for data from actual control of the data by using separate file and storage managers. Each file manager provides access to stored files and stores metadata associated with each of the files for which it is responsible. A file manager control component allocates responsibilities among file managers, assigns management responsibilities for individual files, and maintains a record of which files are assigned to each manager. Each storage manager is responsible for managing storage of files on data storage devices and maintains a record of the location of each stored file. A storage manager control component allocates responsibilities among storage managers, assigns responsibilities for individual files, and maintains a record of which files and storage devices are assigned to each manager.

Abstract: Methods are provided for the utilization of bacterial cell-free extracts in the synthesis of high yields of membrane-associated polypeptides.

Abstract: In an illustrative implementation of this invention, a mechanical computer is manufactured without a separate step of assembly. This is achieved by producing parts in a three dimensional pattern (in relation to each other) such that, when this manufacturing is completed, the components are already assembled to form a computer or part of a computer. The second step of assembling the parts into a desired three dimensional pattern is avoided, because the parts, when made, are already arranged in that 3D pattern.

Abstract: In an exemplary embodiment of this invention, corrugated board is folded into a building system. The building system comprises a plurality of blocks and connectors. The blocks are rectangular cuboid in shape. The bottom and top sides of the blocks have rectangular arrays of holes. The blocks may be releasably connected by aligning the top holes of one block with the bottom holes of another block, and then inserting connectors into the aligned pairs of holes, one connector per aligned paid of holes. The ends of the connectors that are inserted are circular or equiangular polygonal in shape. In some embodiments, compression is used to releasably hold the connectors in place.

Abstract: Bacterial immunity proteins are utilized to increase immune response to an antigen of interest.

Abstract: In illustrative implementations of this invention, a mechanical clock is produced in a manner such that, once its separate parts are made, the clock requires no further assembly. In one implementation of this invention, a mechanical clock is designed using standard CAD (computer-aided design) modeling tools and made with a rapid prototyping machine. The support material used in rapid prototyping is then removed. Once the support material is removed, the clock runs without any additional assembly. In this implementation, the clock contains all the components that are required and they are all constructed in the rapid prototyping process.