Abstract: A dual particle immunoassay method and kit for detecting analyte in a sample in which the sample to be analyzed, a binding molecule specific for the analyte, and a particle coated with the analyte to be detected or coated with a second binding molecule are reacted and applied to a porous membrane. The competitive immunoassay utilizes an analyte-coated particle, whereas the sandwich immunoassay employs a second binding molecule-coated particle. All of the reagents except for the coated particle are able to pass through the porous membrane. Detectable particles coated with a binding substance that binds to the binding molecule, such as protein A protein G, second antibody reactive to the binding molecule, or a small synthetic affinity ligand, are reacted with coated particles retained on the membrane surface. The detectable particles will pass through the membrane if not complexed with the coated particle.

Abstract: Reagents, immunoassay methods, and kits for detecting the presence or amount of trichloroethylene or perchloroethylene in a sample. Reagents include antibodies, immunogens for preparing antibodies, and assay conjugates. The immunogens elicit high affinity antibodies to trichloroethylene. The antibodies have minimal crossreactivity to other halogenated and aromatic hydrocarbons. The assay conjugates provide for sufficient binding of the antibody to the conjugate with maximum inhibition of that binding by free trichloroethylene and perchloroethylene in the sample. The reagents are useful in immunoassays to detect trichloroethylene and perchloroethylene, especially in environmental samples. Kits contain antibodies specific for trichloroethylene and perchloroethylene and assay conjugates.