Abstract: The present invention relates to a method for the formulation of fibrin sealant in a single delivery system. The method involves mixing a fibrinogen/Factor XIII precipitate solution with thrombin under conditions such that thrombin clotting activity is inhibited and said mixture is applied to a body site under conditions which activate the thrombin to convert fibrinogen into fibrin sealant. A single device, syringe or container, can be used to apply the fibrin sealant formulation.

Abstract: The present invention relates to a method for the formulation of fibrin sealant in a single delivery system. The method involves mixing a fibrinogen/Factor XIII precipitate solution with thrombin under conditions such that thrombin clotting activity is inhibited and said mixture is applied to a body site under conditions which activate the thrombin to convert fibrinogen into fibrin sealant. A single device, syringe or container, can be used to apply the fibrin sealant formulation.

Abstract: A device for use in cryopreservation of blood vessels comprising a pair of stylets insertable into the ends of a dissected blood vessel segment. The stylets are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.

Abstract: A device for use in cryopreservation of blood vessels comprising a pair of stylets insertable into the ends of a dissected blood vessel segment. The stylets are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant and DMSO is disclosed as a penetrating cyroprotectant.

Abstract: A device for use in cryopreservation of blood vessels comprising a pair of styles insertable into the ends of a dissected blood vessel segment. The styles are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.

Abstract: A device for use in cryopreservation of blood vessels comprising a pair of stylets insertable into the ends of a dissected blood vessel segment. The stylets are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.

Abstract: A method of freezing, storing and thawing collagen-rich tissue, such as heart valves. The method includes a freezing profile for freezing the tissue down to the temperature of liquid nitrogen with minimal tissue damage due to ice crystal formation. Heart valves cryopreserved according to the present process exhibit a high cell viability when thawed and are suitable for implanting in human patients.

Abstract: A reliable and inexpensive method has been developed to continually regulate the blood pressure of mammals at virtually any desired level. The pressure thus regulated may be maintained within 10 mm Hg tolerances. The method may be carried out by a closed loop apparatus with which (1) the actual blood pressure is continually measured, (2) a function of the actual blood pressure is compared with a desired blood pressure, (3) any difference between the blood pressure expressed by the function of the actual blood pressure and the desired blood pressure is converted into an error signal, (4) the error signal is converted into proportional and derivative functions which in turn (5) control the rate of pumping of a supply of a liquid hypertensive agent or a hypotensive agent into the circulatory system of the mammal.