Abstract: Described herein are compositions and methods for preparing double-stranded complementary DNA (cDNA) from RNA. In some embodiments, these methods allow isothermal preparation of cDNA. In some embodiments, these methods allow mesophilic or thermostable preparation of cDNA. Also described herein are compositions and methods for preparing cDNA and a library of double-stranded cDNA fragments in a single reaction vessel.

Abstract: In an approach to generating a wake-up advertisement to a remote device, a system includes: a computing device comprising communications circuitry, including an advertisement initiation circuitry; one or more computer processors; one or more non-transitory computer readable storage media; and instructions stored on the one or more non-transitory computer readable storage media for execution by at least one of the one or more computer processors. The instructions include: send one or more wake-up advertisements to one or more remote devices; receive one or more connection advertisements from the responding remote device; and responsive to receiving the one or more connection advertisements from the responding remote device, connect to the responding remote device.

Abstract: In an approach to networking devices using an ephemeral mesh network, the system includes: a plurality of devices, each device of the plurality of devices further comprising a wireless interface to communicate with a mobile device; and a controller. The controller includes circuitry configured to connect to the mobile device; authenticate the mobile device; responsive to authenticating the mobile device, establishing a mesh network of the plurality of devices, wherein each light device connects to at least one other devices of the plurality of devices to create a mesh network; and responsive to disconnecting from the mobile device, dissolving the mesh network by disconnecting each device from the plurality of devices.

Abstract: Programmable control devices that rely on time-of-day accuracy maintain that accuracy by determining their location via a global positioning system, recording and timestamping continually measured ambient light via a daylight sensor and real-time clock (RTC) of the programmable control device, determining a mid-darkness time point, comparing that mid-darkness time point with a reference mid-darkness time point for the same location for the same day of the year, and then adjusting the RTC of the programmable control device as necessary. Methods of maintaining time of day in a programmable control device are also provided, as are other aspects.