Patents by Inventor Robert W. Kwiatkowski
Robert W. Kwiatkowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8765418Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: February 15, 2013Date of Patent: July 1, 2014Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8598338Abstract: The present disclosure provides methods for isolating substantially pure and undegraded DNA from biological material via a solid support. The methods may use a DNA lysing solution that comprises a surfactant, a DNA-complexing salt (e.g., a lithium salt), and a buffer. Alternatively, the methods may use a solid support pre-treated with the DNA lysing solution.Type: GrantFiled: March 5, 2010Date of Patent: December 3, 2013Assignee: QIAGEN North American Holdings, Inc.Inventors: Robert Jackson Bair, Kristen Campbell Benedict, Wendy J. Kivens, Robert W. Kwiatkowski, Jr., Kim Paulsen, Daniel A. Strom, John M. Wages
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Publication number: 20130149697Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: ApplicationFiled: February 15, 2013Publication date: June 13, 2013Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, JR., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8389245Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: September 19, 2007Date of Patent: March 5, 2013Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8063184Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: October 5, 2010Date of Patent: November 22, 2011Assignee: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, Jr., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Publication number: 20110111397Abstract: The present invention provides compositions and methods for the detection and characterization of mutations associated with non-syndromic hearing impairment. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the Connexin 26, or gap junction beta 2, gene associated with non-syndromic hearing loss.Type: ApplicationFiled: April 20, 2010Publication date: May 12, 2011Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Andrea L. Mast, Erin Dorn, Robert W. Kwiatkowski, JR., Molly Accola, Susan S. Wigdal
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Publication number: 20110104682Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: October 5, 2010Publication date: May 5, 2011Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, JR., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Patent number: 7935800Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: December 19, 2006Date of Patent: May 3, 2011Assignee: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y Takova, Lisa C. Thompson, Kevin L. Vedvik
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Patent number: 7932039Abstract: The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.Type: GrantFiled: January 27, 2009Date of Patent: April 26, 2011Assignee: Third Wave Technologies, Inc.Inventors: Poonam Agarwal, Robert W. Kwiatkowski, Jr.
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Patent number: 7807806Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: GrantFiled: October 30, 2007Date of Patent: October 5, 2010Assignee: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, Wupo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Publication number: 20100160619Abstract: Reagents, methods and kits for the purification of DNA from biological materials are provided.Type: ApplicationFiled: March 5, 2010Publication date: June 24, 2010Applicant: Qiagen North American Holdings, Inc.Inventors: Robert Jackson Bair, Kristen Campbell Benedict, Wendy J. Kivens, Robert W. Kwiatkowski, JR., Kim Paulsen, Daniel A. Strom, John M. Wages
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Patent number: 7700750Abstract: The present invention provides compositions and methods for the detection and characterization of mutations associated with non-syndromic hearing impairment. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the Connexin 26, or gap junction beta 2, gene associated with non-syndromic hearing loss.Type: GrantFiled: January 9, 2004Date of Patent: April 20, 2010Assignee: Third Wave Technologies, Inc.Inventors: Andrea L. Mast, Erin Dorn, Robert W. Kwiatkowski, Jr., Molly Accola, Susan S. Wigdal
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Publication number: 20090203018Abstract: The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.Type: ApplicationFiled: January 27, 2009Publication date: August 13, 2009Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Poonam Agarwal, Robert W. Kwiatkowski, JR.
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Publication number: 20090142754Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: October 30, 2007Publication date: June 4, 2009Applicant: Third Wave Technologies, Inc.Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Patent number: 7527948Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: September 27, 2004Date of Patent: May 5, 2009Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 7514220Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.Type: GrantFiled: October 28, 2007Date of Patent: April 7, 2009Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow, Robert W. Kwiatkowski, Stephanie H. Vavra
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Patent number: 7485443Abstract: The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5? nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.Type: GrantFiled: July 17, 2002Date of Patent: February 3, 2009Assignee: Northwestern UniversityInventors: Priscilla Wilkins Stevens, David Kelso, Victor Lyamichev, Jeff Hall, Bruce P. Neri, Robert W. Kwiatkowski, Jr., Lloyd Smith
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Publication number: 20080293046Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.Type: ApplicationFiled: December 19, 2006Publication date: November 27, 2008Applicant: Third Wave Technologies, Inc.Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, JR., Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
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Publication number: 20080176215Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: ApplicationFiled: September 19, 2007Publication date: July 24, 2008Applicant: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 7381530Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.Type: GrantFiled: July 23, 2004Date of Patent: June 3, 2008Assignee: Third Wave Technologies, Inc.Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow, Robert W. Kwiatkowski, Stephanie H. Vavra