Abstract: Disclosed is an in vivo method of incorporating exogenous genetic material into the genome of a vertebrate, which involves administering to a male vertebrate's testis a gene delivery mixture comprising a viral vector, such as a retroviral vector, to deliver a polynucleotide encoding a desired trait or product. Also disclosed is an in vitro method of incorporating exogenous genetic material into the genome of a vertebrate, in which germ cells are obtained from a donor male vertebrate and are genetically modified in vitro, before being transferred to a recipient male vertebrate. After the transfer, the male vertebrate bearing the genetically modified germ cells is bred with a female vertebrate such that a transgenic progeny is produced that carries the polynucleotide in its genome. Also disclosed are non-human transgenic vertebrates produced in accordance with the method, including transgenic progeny.

Abstract: A method for locating a machine includes collecting machine data from a first machine over a communications network. The machine data for the first machine includes data reflecting at least one operational parameter associated with the first machine. The method also includes determining that the first machine is missing based on the collected machine data and receiving, from the first machine, over the communications network, a location message including location data reflecting a geographic location of the first machine. The method further includes sending a notification message to a first entity including data reflecting a status of the first machine and the location data, and sending a command to the first machine to control at least one operation of the first machine based on determining that the first machine is missing.

Abstract: A data communication system for a component of a machine includes a control module configured to monitor at least one type of machine data associated with the machine component configured to be mounted on the machine. The data communication system also includes a processor device configured to receive and process the machine data from the control module, and a transceiver device configured to communicate with the processor device and at least one off-board system. The processor device, the transceiver device, and the control module are formed integral with the machine component such that the data communication system communicates the machine data to the at least one off-board system when the machine component is not mounted on the machine.

Abstract: Disclosed is an in vivo method of incorporating exogenous genetic material into the genome of a vertebrate, which involves administering to a male vertebrate's testis a gene delivery mixture comprising a viral vector, such as a retroviral vector, to deliver a polynucleotide encoding a desired trait or product. Also disclosed is an in vitro method of incorporating exogenous genetic material into the genome of a vertebrate, in which germ cells are obtained from a donor male vertebrate and are genetically modified in vitro, before being transferred to a recipient male vertebrate. After the transfer, the male vertebrate bearing the genetically modified germ cells is bred with a female vertebrate such that a transgenic progeny is produced that carries the polynucleotide in its genome. Also disclosed are non-human transgenic vertebrates produced in accordance with the method, including transgenic progeny.

Abstract: An aircraft including a fuselage and at least one wing which may vary incidence with respect to the fuselage for the purpose of extremely short take off and landing and an air cushion system mounted beneath the fuselage for the purpose of surface independent taxi, take off, and landing. The wings may be straight, forward swept, swept, or have the ability to change shape and sweep based on the conditions of flight. The air cushion system may use engine thrust for lift or may use a separate lift system, which is specifically used for the air cushion. The air cushion skirt may also be rigid, non-rigid, or semi-rigid, but shall incorporate a flotation and support system, to allow flotation on water and parking of the aircraft on solid surfaces.

Abstract: Disclosed is an in vivo method of incorporating exogenous genetic material into the genome of a non-human vertebrate, including but not limited to a non-human mammal.

Abstract: A composition for in vitro and in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A method of genetically altering a vertebrate male germ cell in vivo employs a lentiviral-derived vector. A method of substantially depopulating a vertebrate testis employs a combination of a dose of an alkylating agent, such as busulfan, chlorambucil, cyclophosphamide, melphalan, or ethyl ethanesulfonic acid, and a dose of gamma radiation. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate.

Abstract: A composition for in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

Abstract: A composition for in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

Abstract: A composition for in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

Abstract: A composition for in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

Abstract: Disclosed is a method of obtaining selectable transgenic stem cells of a vertebrate by transfecting a male germ cell with a transfection mixture comprising a nucleic acid construct containing a transcriptional unit of a stem cell-specific promoter, for example, a cyclin A1 promoter, operatively linked to a gene encoding a fluorescent or light-emitting reporter protein. The transfection mixture is a composition for transfection, in vivo or ex vivo, of a vertebrate's male germ cells, which comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell.

Abstract: Disclosed is a method of obtaining selectable transgenic stem cells of a vertebrate by transfecting a male germ cell with a transfection mixture comprising a nucleic acid construct containing a transcriptional unit of a stem cell-specific promoter, for example, a cyclin A1 promoter, operatively linked to a gene encoding a fluorescent or light-emitting reporter protein. The transfection mixture is a composition for transfection, in vivo or ex vivo, of a vertebrate's male germ cells, which comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell.

Abstract: Systems and apparatus are provided for treating waste-containing water such as toilet waste. In one aspect, an apparatus for secondary treatment of effluent from a water treatment process, includes a medium-filled tank adapted for gravity flow of the effluent through a plurality of discrete zones formed in the medium, wherein effluent flow through zones is alternately upward or downward. Further, a waste treatment system includes a primary tank for receiving undiluted waste and a medium-filled secondary tank for receiving gravity feed of effluent from the primary tank, wherein the secondary tank is adapted for gravity flow of the effluent through a plurality of discrete zones formed in the medium, and wherein effluent flow through zones is alternately upward or downward.

Abstract: A composition for in vivo transfection of non-human mammalian male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into non-human mammalian male germ cells comprises the administration of the composition to a non-human mammalian. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.

Abstract: The present invention relates to ophthalmic lenses with a refractive index from 1.498 to 1.505, which are the cured product of compositions comprising 60-99 wt. % of at least one poly(allyl carbonate) of a polyhydroxy alcohol, said polyhydroxy alcohol having from 2 to 20 carbon atoms and from 2 to 6 hydroxy groups in the molecule, 0.05 to 5 wt. % of at least one diallyl phthalate type oligomer, 0-20 wt. % of comonomers, and 0.01 to 10 wt. % of a radical initiator. These ophthalmic lenses have the advantage of reduction of mould damage during their preparation without any other lens property, such as yellowness index and barcol hardness, undergoing significant change. The present invention also relates to the production of ophthalmic lenses and the use of diallyl phthalate type oligomers in ophthalmic lenses.

Abstract: An automatic adjusting threshold microphone squelch system for use in aircraft or other high noise environments including digital drop-out timer and voice detector connected directly to an analog composite signal processor. The processor is comprised of two channels including a microphone audio channel and an aircraft noise detection channel. The channels, driven from a common microphone, are arranged in parallel with the noise detector feeding its noise-dependent output forwardly to a variable-threshold microphone audio post-amplifier. The composite audio from this amplifier is automatically maintained below a first digital "zero" level when voice is absent while containing peaks above a second digital "one" level when audio is present. This composite audio directly feeds the digital detector/timer without further wave-shaping or other signal processing.

Abstract: The present invention relates to ophthalmic lenses with a refractive index from 1.50 to 1.51, which are the cured product of compositions comprising 60-99% wt % of at least one poly(allyl carbonate) of a polyhydroxy alcohol, said polyhydroxy alcohol having from 2 to 20 carbon atoms and from 2 to 6 hydroxy groups in the molecule, less than comonomers, and 0.01 to 10 wt % of a radical initiator. These ophthalmic lenses have the advantages of improved tintability and a low rejection rate in their production due to the reduction in prerelease during polymerization casting. Preferably, the ophthalmic lenses and the use of diallyl phthalate type oligomers in ophthalmic lenses.