Abstract: The present disclosure features methods, cells or cell lines, and compositions for increasing the amount of products, e.g., proteins, produced by a cell or cell line by reducing the level of a non-essential endogenous protein.

Abstract: A dental handpiece includes a head portion supporting a turbine for a removable rotating dental tool, and a handle portion having an internal cavity. One or more conduits extend at least partially through the internal cavity toward the head portion. At least one of the conduits is a drive fluid tube delivering drive fluid to the head portion for rotating the turbine. A wireless communication device within the internal cavity is attached to at least one of the conduits. At least a section of the handle portion surrounding the wireless communication device is formed of a dielectric material. The wireless communication device is configured to wirelessly communicate with an external reader or writer and store data related to at identification, use, and/or maintenance of the handpiece. An electrically inert substrate material encapsulates the wireless communication device, which is electrically isolated from other components of the handpiece.

Abstract: The present disclosure features methods, cells or cell lines, and compositions for increasing the amount of products, e.g., proteins, produced by a cell or cell line by reducing the level of a non-essential endogenous protein.

Abstract: A system for monitoring anxiety of an individual includes a chair having at least a seat and a back, a base supporting the chair, and a first sensor disposed in at least one of the seat or the back. The first sensor is configured to, without being in direct contact with the individual, continuously obtain data related to a first physical characteristic from the individual seated in the chair. The first physical characteristic is one of respiration or pulse rate. The system further includes a display and a controller. The controller is configured to receive, in real-time, the data related to the first physical characteristic from the first sensor and compile a first metric from the received data. The first metric is configured to visually represent a change in the first physical characteristic. The controller is further configured to output the first metric to the display in real-time.

Abstract: Provided are TYK2 inhibitors of Formula I, processes for their production, their use as pharmaceuticals and pharmaceutical compositions comprising them. These compounds are useful, for example, in treating TYK2-mediated disorders, such as, autoimmune and inflammatory disorders, metabolic disorders, proliferative disorders, endocrine disorders, neurological disorders, allergic disorders, and disorders associated with transplantation.

Abstract: A dental tool tip is removably attached to a vibratory dental handpiece. The dental tool tip includes a mounting base configured for removable engagement with the dental handpiece, the mounting base having an open-ended, internal bore extending therethrough and configured for fluid communication with the dental handpiece and a body having a free distal, terminal end and at least a portion of the body having an abrasive coating. A mounting rod proximally extends from the body and is at least partially received within the internal bore. The mounting rod has at least one open-ended passage extending therethrough and in registry and fluid communication with the internal bore, thereby being configured to enable at least one of air or water to advance from the dental handpiece, and flow out onto the body, via the internal bore and the at least one channel.

Abstract: Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

Abstract: Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

Abstract: In one embodiment, an animal monitoring system is described. The animal monitoring system may comprise a monitor unit attached to an animal collar. The monitor unit may comprise one or more sensors for detecting health and activity information regarding an animal. The monitor unit may be configured to wirelessly transmit health and activity information relating to the animal via a wireless network to an internet server that is accessible via a remote device. The remote device may comprise a computer, cell phone, laptop, etc. The health and activity information may include information such as the animal's heart rate, respiratory rate, activity level, temperature, blood oxygen levels, electrocardiogram, blood glucose, step count, calories burned, sleep patterns, and location. In one embodiment, the animal monitoring system further comprises a radar-based sensor system configured to use radar to detect health and activity information about the animal.

Abstract: Disclosed are genetic control circuits, cells, and methods that use a repressor polypeptide to reduce the transcription rate of an exogenous therapeutic polypeptide encoding gene in response to a change in condition.

Abstract: Provided herein are uses of the compounds for the treatment of various indications, including prostate cancer and Kennedy's disease.

Abstract: The present invention relates to an expression system for the heterologous expression of a nucleic acid sequence of interest in a mammalian cell, the system comprising: (i) a first genetic entity, comprising: a nucleic acid sequence encoding a functional Epstein Barr virus nuclear antigen 1 (EBNA-1), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional EBNA-1; (ii) a second genetic entity, comprising: a nucleic acid sequence encoding a functional nucleoside diphosphate kinase A (NDPK-A), the nucleic acid sequence being operably linked to regulatory elements that allow for expression of the nucleic acid sequence encoding a functional NDPK-A; (iii) a third genetic entity, comprising: the nucleic acid sequence of interest being operably linked to regulatory elements that allow for expression of the nucleic acid sequence of interest; and (iv) a four genetic entity, comprising: the Epstein Barr virus OriP sequence or one

Abstract: Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

Abstract: A method for maintaining a fluid containment asset is described. The method comprises: configuring a first schedule having a first work task on the fluid containment asset for a first project; performing the first work task on the fluid containment asset based on the first schedule; measuring a work task performance for the first work task performed based on the first schedule; collecting first work task data corresponding to the first work task performed; building a first predictive model based on at least the first work task data; configuring a second schedule using the first predictive model, wherein the second schedule has a second work task for a second project; and performing the second work task on the fluid containment asset based on the second schedule.

Abstract: A dental handpiece includes a head portion supporting a turbine for a removable rotating dental tool, and a handle portion having an internal cavity. One or more conduits extend at least partially through the internal cavity toward the head portion. At least one of the conduits is a drive fluid tube delivering drive fluid to the head portion for rotating the turbine. A wireless communication device within the internal cavity is attached to at least one of the conduits. At least a section of the handle portion surrounding the wireless communication device is formed of a dielectric material. The wireless communication device is configured to wirelessly communicate with an external reader or writer and store data related to at identification, use, and/or maintenance of the handpiece. An electrically inert substrate material encapsulates the wireless communication device, which is electrically isolated from other components of the handpiece.

Abstract: A plurality of accessory components for use in respective reel to reel sectioning, serial sectioning, and array tomography sectioning described herein incorporate mechanical and electro-mechanical engineering to provide accessory tools for attachment to and use with a microtome, as shown in several of the figures of this disclosure.

Abstract: The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.

Abstract: A method of controlling a gas mixture for a laser includes receiving as an input one or more of a plurality of concentration values. Each of the concentration values respectively corresponds to a constituent of a plurality of constituents of air. In the method, a blend of the plurality of constituents of air is generated based on the received one or more of the concentration values. The method also includes determining whether the blend of the plurality of constituents of air is within a threshold range for a ratio of the concentration values for the plurality of constituents of air. A flow of the blend of the plurality of constituents of air is controlled to be routed through an output circuit for use as the gas mixture for the laser following a determination that the blend of the plurality of constituents of air is within the threshold range.