Patents by Inventor Roberto E. Armenta
Roberto E. Armenta has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10907115Abstract: Provided herein are methods for winterizing oil. The methods include heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time; reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil; removing the first solid fraction from the oil; reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil; removing the second solid fraction from the oil; and recovering the second liquid fraction of the oil.Type: GrantFiled: July 31, 2018Date of Patent: February 2, 2021Assignee: MARA RENEWABLES CORPORATIONInventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Publication number: 20210024852Abstract: Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.Type: ApplicationFiled: August 10, 2020Publication date: January 28, 2021Inventors: Dorothy A. Dennis, Roberto E. Armenta
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Patent number: 10851395Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.Type: GrantFiled: June 10, 2016Date of Patent: December 1, 2020Assignee: MARA Renewables CorporationInventors: Zhiyong Sun, Alan Sothern, Kevin Berryman, Mercia Valentine, Michael Milway, Laura Purdue, Roberto E. Armenta
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Publication number: 20200332338Abstract: Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.Type: ApplicationFiled: April 30, 2020Publication date: October 22, 2020Inventors: Zhiyong Sun, Michael Milway, Kevin Berryman, Mercia Valentine, Roberto E. Armenta, Laura Purdue
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Publication number: 20200325465Abstract: Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.Type: ApplicationFiled: April 30, 2020Publication date: October 15, 2020Inventors: Alexandra Merkx-Jacques, David Woodhall, Mark Scaife, Roberto E. Armenta, Denise Muise, Holly Rasmussen, Jeremy Benjamin
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Patent number: 10745642Abstract: Provided herein are methods of recovering oil from microorganisms. The methods are useful, for example, in obtaining nutritional oils and/or lipid biofuels. The methods of recovering oil described herein include contacting a population of microorganisms with one or more enzymes under conditions that cause disruption of the microorganisms, concentrating the disrupted microorganisms, and extracting lipids from the disrupted microorganisms at high temperature in the presence of a salt and in the absence of solvent.Type: GrantFiled: July 25, 2017Date of Patent: August 18, 2020Assignee: MARA Renewables CorporationInventors: Dorothy A. Dennis, Roberto E. Armenta
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Patent number: 10676709Abstract: Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.Type: GrantFiled: July 20, 2017Date of Patent: June 9, 2020Assignee: MARA Renewables CorporationInventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Patent number: 10676775Abstract: Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.Type: GrantFiled: October 14, 2015Date of Patent: June 9, 2020Assignee: MARA Renewables CorporationInventors: Zhiyong Sun, Michael Milway, Kevin Berryman, Mercia Valentine, Roberto E. Armenta, Laura Purdue
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Patent number: 10662418Abstract: Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.Type: GrantFiled: March 12, 2018Date of Patent: May 26, 2020Assignee: Mara Renewables CorporationInventors: Alexandra Merkx-Jacques, David Woodhall, Mark Scaife, Roberto E. Armenta, Denise Muise, Holly Rasmussen, Jeremy Benjamin
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Patent number: 10385370Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.Type: GrantFiled: March 20, 2018Date of Patent: August 20, 2019Assignee: MARA Renewables CorporationInventors: Zhiyong Sun, Alan Sothern, Kevin Berryman, Mercia Valentine, Michael Milway, Laura Purdue, Roberto E. Armenta
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Publication number: 20190093043Abstract: Provided herein are methods for winterizing oil. The methods include heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time; reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil; removing the first solid fraction from the oil; reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil; removing the second solid fraction from the oil; and recovering the second liquid fraction of the oil.Type: ApplicationFiled: July 31, 2018Publication date: March 28, 2019Applicant: Mara Renewables CorporationInventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Publication number: 20180291363Abstract: Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.Type: ApplicationFiled: March 12, 2018Publication date: October 11, 2018Applicant: MARA Renewables CorporationInventors: Alexandra Merkx-Jacques, David Woodhall, Mark Scaife, Roberto E. Armenta, Denise Muise, Holly Rasmussen, Jeremy Benjamin
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Patent number: 10059906Abstract: Provided herein are methods for winterizing oil. The methods include heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time; reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil; removing the first solid fraction from the oil; reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil; removing the second solid fraction from the oil; and recovering the second liquid fraction of the oil.Type: GrantFiled: July 20, 2017Date of Patent: August 28, 2018Inventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Publication number: 20180208954Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.Type: ApplicationFiled: March 20, 2018Publication date: July 26, 2018Inventors: Zhiyong Sun, Alan Sothern, Kevin Berryman, Mercia Valentine, Michael Milway, Laura Purdue, Roberto E. Armenta
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Publication number: 20180179560Abstract: Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.Type: ApplicationFiled: December 18, 2017Publication date: June 28, 2018Inventors: Violeta Ugalde, Zachary Sun, Anthony Windust, Roberto E. Armenta
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Patent number: 9951326Abstract: Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganisms include at least one nucleic acid sequence encoding a xylulose kinase and/or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganisms are capable of growing on xylose as a carbon source.Type: GrantFiled: July 13, 2016Date of Patent: April 24, 2018Inventors: Alexandra Merkx-Jacques, David Woodhall, Mark Scaife, Roberto E. Armenta, Denise Muise, Holly Rasmussen, Jeremy Benjamin
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Publication number: 20180023032Abstract: Provided herein are methods for winterizing oil. The methods include heating the oil to a first temperature and maintaining the oil at the first temperature for a first period of time; reducing the first temperature of the oil after the first period of time to a second temperature over a second period of time, wherein reducing the first temperature produces a first solid fraction and first liquid fraction of the oil; removing the first solid fraction from the oil; reducing the second temperature of the first liquid fraction of the oil over a third period of time to a third temperature, wherein reducing the second temperature of the oil produces a second solid fraction and second liquid fraction of the oil; removing the second solid fraction from the oil; and recovering the second liquid fraction of the oil.Type: ApplicationFiled: July 20, 2017Publication date: January 25, 2018Applicant: Mara Renewables CorporationInventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Publication number: 20180023047Abstract: Provided herein is a method for obtaining flowable oil comprising the steps of providing a population of oil-producing microorganisms; recovering oil from the microorganisms, wherein the oil is at a first temperature; reducing the first temperature over a first period of time to a second temperature; and applying mechanical energy to the oil during the first period of time thereby producing the flowable oil.Type: ApplicationFiled: July 20, 2017Publication date: January 25, 2018Applicant: Mara Renewables CorporationInventors: Xuan Jiang, Dorothy Dennis, Roberto E. Armenta
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Patent number: 9873880Abstract: Provided are Thraustochytrid and Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences and genes from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, means for mutagenizing microorganisms, strains produced by mutagenesis, and methods and compositions related to production of particular compounds in microorganisms.Type: GrantFiled: March 12, 2014Date of Patent: January 23, 2018Assignee: DSM Nutritional Products AGInventors: Mark A. Scaife, Roberto E. Armenta
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Publication number: 20170356018Abstract: Provided herein are methods of producing oils with reduced saturated fatty acids. The methods include culturing oil-producing microorganisms in a fermentation medium in the presence of one or more antifoaming agents under a controlled carbon consumption rate, wherein the culturing produces oils comprising fatty acids and wherein less than 35% of the fatty acids in the oil are saturated fatty acids.Type: ApplicationFiled: June 10, 2016Publication date: December 14, 2017Inventors: Zhiyong Sun, Alan Sothern, Kevin Berryman, Mercia Valentine, Michael Milway, Laura Purdue, Roberto E. Armenta