Abstract: A process for producing optically active (R)-3-hydroxybutyrate and (R)-3-hydroxyvalerate mixtures, where the ratio between them is defined by the composition of PHB-co-HV, used as raw material for the production process, formulations containing the mixtures, and uses of the formulations. A use or method of treating a subject having a metabolic disorder, comprising administering optically active mixtures of (R)-3-hydroxybutyrate and (R)-3-hydroxyvalerate, their respective salts or esters derived therefrom, obtained according to the process of the present disclosure at a therapeutically effective amount to treat at least one symptom of the metabolic disorder.

Abstract: The present invention refers to a biological formulation for complementing an animal feed, which comprises: dry cells of Cupriavidus necator or Alcaligenes latus bacteria, containing at minimum 40% of PHB and B vitamins, tyrosine, glutamic acid, phosphates and iron, which formulation may further contain the endogenous PHB depolymerase enzyme. The process for obtaining the formulation comprises the steps of: (a) producing, by aerobic fermentation, from 32 to 34° C., in a mineral and aqueous fermentative medium containing sucrose, glucose or other sugary streams derived from sugar production, cells of Cupriavidus necator or Alcaligenes latus bacteria containing at minimum 40% of PHB and B vitamins, tyrosine, glutamic acid, phosphates and iron; (b) inactivating the cells, thermally, at a temperature from 65 to 90° C. for 15 minutes; and (c) drying the bacteria cells obtained in the previous step.

Abstract: A process for recovering polyhydroxyalkanoates (PHAs) from cellular biomass of bacteria, said biomass being obtained by fermentation and in the form of a cellular biomass slurry in aqueous suspension, comprising the steps of: submitting the slurry to operations of injection of PHA solvent, agitation and heating, in order to form a suspension comprising PHA solvent with the dissolved PHA, water and insoluble residues; recuperating the solvent enriched with PHA; rapidly cooling the solution of PHA solvent to precipitate the dissolved PHA; micro-filtrating the suspension of PHA precipitated in the solvent, in order to separate a paste concentrated with precipitated PHA; washing with water, heating and agitating the concentrated PHA paste, to promote evaporation of the solvent and to obtain a suspension containing PHA granules; agitating and shearing the PHA granules and depleting the residual solvent; and separating the purified PHA particles from the suspension.