Abstract: The disclosure relates to the field of molecular biology, to compositions and methods for the usage of bidirectional selection marker systems in microbial cells. Specifically, this disclosure pertains to compositions and methods for reestablishing a predetermined target sequence within a microbial genome, by integrating and excising a bidirectional selection marker construct in consecutive transformation steps. Methods and compositions are also disclosed in which bidirectional selection marker systems are used in multiplex genome engineering, by combining marker integration or excision with simultaneously modifying at least one additional target sequence at a different genome sequence.

Abstract: The disclosure relates to the field of molecular biology, to compositions and methods for the usage of recombinant self-excisable selection marker systems in microbial cells. Specifically, this disclosure pertains to compositions and methods for reestablishing a predetermined target sequence within a microbial genome, by integrating and excising two recombinant self-excisable selection marker constructs in consecutive transformation steps. Methods and compositions are also disclosed in which recombinant self-excisable selection marker systems are used in multiplex genome engineering, by combining marker integration with simultaneously modifying at least one additional target sequence at a different genome sequence.

Abstract: Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

Abstract: Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

Abstract: Provided herein, inter alia, are engineered phytase polypeptides and fragments thereof with improved thermotolerance as well as methods for producing and using the same for enhancing animal performance on one or more metrics.

Abstract: The present invention related to a method for preparing a protein hydrolysate from a proteinaceous material by contacting the material with a proteolytic enzyme mixture having a proline specific exopeptidase. In particular, the proline specific exopeptidase is an aminopeptidase specific for at the five amino acid N-terminal sequence X-Pro-Gln-Glv-Pro-, where X is any amino acid. The present invention also relates to use of the aminopeptidase with a second exopeptidase and an endopeptidase.