Patents by Inventor Rocco L. Policarpo

Rocco L. Policarpo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein retrosplicing enabled by a double ligation reaction
    Patent number: 9731029
    Abstract: Proteins containing a C-terminal thioester are important intermediates in semi-synthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. The invention involves, in some aspects a method, utilizing Sortase A, for preparation of recombinant proteins containing a C-terminal ?thioester. This new method for double ligatation is useful for synthesizing new or naturally occurring molecules such as a protein thioester.
    Type: Grant
    Filed: May 21, 2013
    Date of Patent: August 15, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Bradley L. Pentelute, Xiaoli Liao, Amy E. Rabideau, Jingjing Ling, Rocco L. Policarpo
  • PROTEIN RETROSPLICING ENABLED BY A DOUBLE LIGATION REACTION
    Publication number: 20150152134
    Abstract: Proteins containing a C-terminal thioester are important intermediates in semi-synthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. The invention involves, in some aspects a method, utilizing Sortase A, for preparation of recombinant proteins containing a C-terminal ?thioester. This new method for double ligatation is useful for synthesizing new or naturally occurring molecules such as a protein thioester.
    Type: Application
    Filed: May 21, 2013
    Publication date: June 4, 2015
    Inventors: Bradley L. Pentelute, Xiaoli Liao, Amy E. Rabideau, Jingjing Ling, Rocco L. Policarpo