Abstract: An apparatus and method to effect a sterile interconnection of lines for transferring sterile medical fluids from a supply container to a fluid utilization device or a fluid storage container. A flexible, transparent, sterile connection bag is provided with at least three inlet/outlet ports. Previously sterilized free ends of the incoming and outgoing fluid lines, provided with connectors, are inserted into the bag through two of the ports and sealed therewith. A gas sterilization line is inserted into the bag through the third port. A sterilizing gas, such as ozone, is introduced into the bag to re-sterilize the lines and the connectors. Through visual inspection and external hand manipulation of the bag and its contents, the two lines are joined within the bag by the line connectors. Sterile fluid transfer through the lines is then undertaken.

Abstract: A disposable apparatus for sterilizing fluid and dispensing it includes a bag, a filter element, a conduit element, and a plurality of dispensing elements serially communicating with each other for providing a sterile fluid dispensing path that can be serially opened and closed. Methods are also provided for filling such apparatus in conjunction with a closed environment of an assembly having at least one mixing vessel, a reservoir container, a filling area, a filter element, and a plurality of storage elements interfacing the filling area.