Abstract: A composition is disclosed. The composition includes a cell culture medium; a proteinase; and a growth promoting factor. The doubling time of cells in the medium is the same or lower as compared to the doubling time of the cells in an otherwise identical composition which lacks the proteinase and has a higher concentration of the growth promoting factor. A method is also disclosed. The method includes growing the cells in a composition. The composition includes a cell culture medium; a proteinase; and a growth promoting factor. The doubling time of cells in the medium is the same or lower as compared to the doubling time of the cells in an otherwise identical composition which lacks the proteinase and has a higher concentration of the growth promoting factor.

Abstract: The invention encompasses composition and methods for cell culture and for therapeutic and cosmetic use. The compositions and methods utilize collagenase, e.g., bacterial collagenase, other isolated collagenase, or synthetic collagenase, e.g., recombinant collagenase. One form of collagenase that can be used in some embodiments of the invention is matrix metalloproteinase-1. The compositions and methods of the invention also optionally utilize a cAMP-elevating agent.

Abstract: Methods, compositions, and kits are disclosed for culturing keratinocytes and for the treatment of wounds and burns, as well as cosmetic compositions. The methods and compositions include the use of conditioned medium from the culture of keratinocytes, where the medium may be used to further culture keratinocytes or used in compositions such as keratinocyte culture medium and pharmaceutical and cosmetic compositions.

Abstract: The invention encompasses composition and methods for cell culture and for therapeutic and cosmetic use. The compositions and methods utilize collagenase, e.g., bacterial collagenase, other isolated collagenase, or synthetic collagenase, e.g., recombinant collagenase. One form of collagenase that can be used in some embodiments of the invention is matrix metalloproteinase-1. The compositions and methods of the invention also optionally utilize a cAMP-elevating agent.

Abstract: The invention encompasses composition and methods for cell culture and for therapeutic and cosmetic use. The compositions and methods utilize collagenase, e.g., bacterial collagenase, other isolated collagenase, or synthetic collagenase, e.g., recombinant collagenase. One form of collagenase that can be used in some embodiments of the invention is matrix metalloproteinase-1. The compositions and methods of the invention also optionally utilize a cAMP-elevating agent.