Abstract: Synthetic RNA molecules comprising at least two RNA-binding protein (RBP)-binding motifs, wherein the at least two RBP-binding motifs bind the same first RBP and comprise non-identical sequences are provided. Synthetic RNA molecules comprising an RBP-binding motif that binds two orthogonal RBPs, comprising at least three RBP-binding motifs for three orthogonal RBPs or comprising a first RBP-binding motif, a second RBP-binding motif, a regulatory element and an open reading frame wherein the first and second RBP-binding motifs cooperatively enhance translation of the open reading frame are also provided. Compositions, cells and methods of use or generating the synthetic RNA molecules are also provided.

Abstract: A data storage system and method are provided, as well as systems and methods for fabrication, and writing and reading of data therein. The data storage system includes at least one population of molecular sequences including chains of basic molecular building-blocks, and defining at least one respective data-block encoding data in the data storage system. The data of the data-block is encoded in a sequence S=(?1, ?2, . . . , ?k . . . , ?K-1, ?K) of encoded letters {?k} associated with an alphabet ??{?m}|m=1 to M, which are encoded according to the types of basic molecular building-blocks appearing at k respective location along storage segments of the molecular sequences of the population. The molecular sequences include a number Z of different types of basic molecular building-blocks {En}|n=1 to Z, while the alphabet ? has a size M strictly greater than the number Z of types of building-blocks.

Abstract: Systems comprising a first polynucleotide comprising a regulatory element operatively linked to an open reading frame encoding an invertase enzyme and a second polynucleotide comprising a repressible regulatory element operatively linked to an open reading frame encoding a glucose oxidase enzyme wherein the repressible element inhibits transcription in response to hydrogen peroxide are provided. Cells and compositions comprising a system of the invention, as well as methods using the system of the invention, are also provided, as is artificial honey.

Abstract: RNA-protein granules comprising fusion proteins and RNA are disclosed, as well as uses thereof for treating or preventing viral infection. Soluble fusion proteins comprising an extracellular domain of a human receptor or a fragment thereof and a bacteriophage coat protein, as well as synthetic microcarriers comprising a solid support conjugated to a plurality of viral proteins or fragments thereof are provided. Nucleic acid molecules and vectors encoding the soluble fusion protein, synthetic RNA-protein granules comprising a fusion protein, as well as method using the soluble fusion protein are also provided.

Abstract: Synthetic RNA molecules comprising at least two first RNA-binding protein (RBP)-binding motifs, at least two second RBP-binding motifs and at least two third RBP-binding motifs, wherein the at least two first RBP-binding motifs bind the same first RBP and comprise non-identical sequences are provided. Synthetic RNA molecules comprising at least two RBP-binding motifs, a regulatory element and an open reading frame wherein the RBP-binding motifs individually repress translation and cooperatively enhance translation of the open reading frame are also provided. Methods employing machine learning models to determine variant sequence binding to RBPs are also provided.

Abstract: A molecular data storage system is presented for encoding data-block(s). The system includes one or more populations of molecular sequences, each population encoding a respective one of the data-blocks. Each molecular sequence comprises a data encoding section comprising a sequence of similar predetermined length N of short k-mers, whereby in each population the data encoding sections of all molecular sequences have the similar predetermined length N. The short k-mers serve as data encoding building blocks of the data encoding sections, whereby valid short k-mers serving as data encoding building blocks form a subset of a building-block-set consisting of a number Z of different preselected short k-mers each presenting a unique combination of a number k of bases of a preselected set of bases, characterized in that all the Z types of short k-mers in said building-block-set have a similar predetermined size k?2 (plurality) of bases.

Abstract: Systems comprising a first polynucleotide comprising a regulatory element operatively linked to an open reading frame encoding an invertase enzyme and a second polynucleotide comprising a repressible regulatory element operatively linked to an open reading frame encoding a glucose oxidase enzyme wherein the repressible element inhibits transcription in response to hydrogen peroxide are provided. Cells and compositions comprising a system of the invention, as well as methods using the system of the invention, are also provided, as is artificial honey.

Abstract: A data storage system and method are provided, as well as systems and methods for fabrication, and writing and reading of data therein. The data storage system includes at least one population of molecular sequences including chains of basic molecular building-blocks, and defining at least one respective data-block encoding data in the data storage system. The data of the data-block is encoded in a sequence S=(?1, ?2, . . . , ?k . . . , ?K-1, ?K) of encoded letters {?k} associated with an alphabet ??{?m}|m=1 to M, which are encoded according to the types of basic molecular building-blocks appearing at k respective location along storage segments of the molecular sequences of the population. The molecular sequences include a number Z of different types of basic molecular building-blocks {En}|n=1 to Z, while the alphabet ? has a size M strictly greater than the number Z of types of building-blocks.

Abstract: Synthetic RNA molecules comprising at least two RNA-binding protein (RBP)-binding motifs, wherein the at least two RBP-binding motifs bind the same first RBP and comprise non-identical sequences are provided. Synthetic RNA molecules comprising an RBP-binding motif that binds two orthogonal RBPs, comprising at least three RBP-binding motifs for three orthogonal RBPs or comprising a first RBP-binding motif, a second RBP-binding motif, a regulatory element and an open reading frame wherein the first and second RBP-binding motifs cooperatively enhance translation of the open reading frame are also provided. Compositions, cells and methods of use or generating the synthetic RNA molecules are also provided.

Abstract: Chimeric polypeptides comprising a dihydrotestosterone (DHT) reductase moiety, such as 3 alpha-hydroxysteroid dehydrogenase (3?-HSD), fused to a signal peptide moiety, polynucleotides encoding same, and compositions comprising at least one microorganism cell capable of secreting the chimeric polypeptide, are provided. Further provided are methods and kits for treating, preventing or ameliorating androgen-dependent disorders, including but not limited to androgenic alopecia.

Abstract: Chimeric polypeptides comprising a dihydrotestosterone (DHT) reductase moiety, such as 3 alpha-hydroxysteroid dehydrogenase (3?-HSD), fused to a signal peptide moiety, polynucleotides encoding same, and compositions comprising at least one microorganism cell capable of secreting the chimeric polypeptide, are provided. Further provided are methods and kits for treating, preventing or ameliorating androgen-dependent disorders, including but not limited to androgenic alopecia.