Patents by Inventor Roelof Hendrik Matthijs Kouwen

Roelof Hendrik Matthijs Kouwen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • starter cultures
    Patent number: 11674117
    Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: June 13, 2023
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell, Pieter Emiel Verloren Van Themaat, Jennifer Mahony
  • Phage insensitive
    Patent number: 10519519
    Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: December 31, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
  • Bacteriophage detection
    Patent number: 10519516
    Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: December 31, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell
  • STREPTOCOCCUS THERMOPHILUS STARTER CULTURES
    Publication number: 20190367866
    Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.
    Type: Application
    Filed: December 14, 2017
    Publication date: December 5, 2019
    Inventors: Roelof Hendrik Matthijs KOUWEN, Douwe VAN SINDEREN, Brian MCDONNELL, Pieter Emiel VERLOREN VAN THEMAAT, Jennifer MAHONY
  • Non-CRISPR-mediated phage resistant
    Patent number: 10244771
    Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: April 2, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Laurens Leendert Hanemaaijer, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
  • PHAGE INSENSITIVE STREPTOCOCCUS THERMOPHILUS
    Publication number: 20180305777
    Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
    Type: Application
    Filed: July 2, 2018
    Publication date: October 25, 2018
    Inventors: Roelof Hendrik Matthijs KOUWEN, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
  • Phage insensitive
    Patent number: 10041135
    Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: August 7, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Roelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
  • BACTERIOPHAGE DETECTION
    Publication number: 20170327912
    Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.
    Type: Application
    Filed: May 8, 2017
    Publication date: November 16, 2017
    Inventors: Roelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell
  • PHAGE INSENSITIVE STREPTOCOCCUS THERMOPHILUS
    Publication number: 20170218468
    Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
    Type: Application
    Filed: February 20, 2015
    Publication date: August 3, 2017
    Inventors: Roelof Hendrik Matthijs KOUWEN, Pim VAN HEE, Douwe VAN SINDEREN, Brian MCDONNELL, Jennifer MAHONY
  • PHAGE RESISTANT LACTIC ACID BACTERIA
    Publication number: 20170196233
    Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.
    Type: Application
    Filed: July 23, 2015
    Publication date: July 13, 2017
    Inventors: Roelof Hendrik Matthijs KOUWEN, Laurens Leendert HANEMAAIJER, Douwe VAN SINDEREN, Brian MCDONNELL, Jennifer MAHONY
  • Host cells and methods of producing disulfide bond containing proteins
    Patent number: 8852886
    Abstract: The invention provides for genetically modified Bacillus host cells having decreased cytoplasmic reductase activity, such as decreased TrxA activity, and a nucleic acid encoding a heterologous Staphylococcal oxidase. The invention also provides for methods of producing di-sulfide bond containing proteins using the host cells of the invention and methods of improving protein folding using the host cell of the invention.
    Type: Grant
    Filed: March 24, 2009
    Date of Patent: October 7, 2014
    Assignee: Danisco US Inc.
    Inventors: Jean-Yves Francois Dubois, Roelof Hendrik Matthijs Kouwen, Jan Maarten Van Dijl
  • Host Cells and Methods Of Producing Disulfide Bond Containing Proteins
    Publication number: 20110236926
    Abstract: The invention provides for genetically modified Bacillus host cells having decreased cytoplasmic reductase activity, such as decreased TrxA activity, and a nucleic acid encoding a heterologous Staphylococcal oxidase. The invention also provides for methods of producing di-sulfide bond containing proteins using the host cells of the invention and methods of improving protein folding using the host cell of the invention.
    Type: Application
    Filed: March 24, 2009
    Publication date: September 29, 2011
    Inventors: Jean-Yves Francois Dubois, Roelof Hendrik Matthijs Kouwen, Jan Maarten Van Dijl