Abstract: We provide isolated TPP and preQ1 class II riboswitches which are labelled for FRET studies of ribosome function. The riboswitches may be used in assays to determine riboswitch function, and to test the activity of compounds in modulating riboswitch function.

Abstract: The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.

Abstract: We provide isolated TPP and preQ1 class II riboswitches which are labelled for FRET studies of ribosome function. The riboswitches may be used in assays to determine riboswitch function, and to test the activity of compounds in modulating riboswitch function.

Abstract: Dye compounds of the formula (1) wherein A is a protective agent group that has a characteristic of modifying the singlet-triplet occupancy of the shown cyanine moiety, and M is a reactive crosslinking group or a group that can be converted to a reactive crosslinking group. Methods for synthesizing the dye compounds and applications for their use are also described.

Abstract: The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.

Abstract: This invention relates to a method to rapidly find crystallization conditions for a biomolecule in a desired conformation using single-molecule, fluorescent resonance energy transfer (smFRET) imaging techniques. The method provides significant cost and time advantages over the empricial exploration for crystallization conditions.

Abstract: The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.

Abstract: Dye compounds of the formula (1) wherein A is a protective agent group that has a characteristic of modifying the singlet-triplet occupancy of the shown cyanine moiety, and M is a reactive crosslinking group or a group that can be converted to a reactive crosslinking group. Methods for synthesizing the dye compounds and applications for their use are also described.

Abstract: This invention relates to a method to rapidly find crystallization conditions for a biomolecule in a desired conformation using single-molecule, fluorescent resonance energy transfer (smFRET) imaging techniques. The method provides significant cost and time advantages over the empricial exploration for crystallization conditions.

Abstract: A method of applying pulsation energy to an online battery backup system including the steps of sampling at least one voltage sampling circuit to monitor a float voltage drop across the terminals of each battery unit within a plurality of battery units, selecting from among the plurality of battery units the unit having the greatest float voltage drop, operating a pulse generation circuit to apply pulsation energy across the terminals of only the selected battery unit, and ceasing to operate the pulse generation circuit in response to a predetermined trigger. A generally corresponding method may be performed on each battery cell within the plurality of battery units. Also, battery pulsation systems for an online battery backup system may include a pulse generation circuit and a controller for selectively applying pulsation energy across the terminals of a selected battery unit or battery cell.

Abstract: A method of applying pulsation energy to an online battery backup system including the steps of sampling at least one voltage sampling circuit to monitor a float voltage drop across the terminals of each battery unit within a plurality of battery units, selecting from among the plurality of battery units the unit having the greatest float voltage drop, operating a pulse generation circuit to apply pulsation energy across the terminals of only the selected battery unit, and ceasing to operate the pulse generation circuit in response to a predetermined trigger. A generally corresponding method may be performed on each battery cell within the plurality of battery units. Also, battery pulsation systems for an online battery backup system may include a pulse generation circuit and a controller for selectively applying pulsation energy across the terminals of a selected battery unit or battery cell.

Abstract: A method of applying pulsation energy to an online battery backup system including the steps of (1) sampling at least one voltage sampling circuit to monitor a float voltage drop across the terminals of each battery unit within a plurality of battery units; (2) selecting from among the plurality of battery units the unit having the greatest float voltage drop; (3) operating a pulse generation circuit to apply pulsation energy across the terminals of only the selected battery unit; and (4) ceasing to operate the pulse generation circuit in response to a predetermined trigger. A generally corresponding method may be performed on each battery cell within the plurality of battery units. Also, battery pulsation systems for an online battery backup system including means for applying pulsation energy across the terminals of only a selected battery unit or a selected battery cell.

Abstract: A method for cooling a battery after a charging phase including the steps of providing a user display, identifying a starting point at which the charging phase of the battery transitions to a cooling phase, when the starting point is identified, instructing the user display to provide a first indication for a first predetermined amount of time, and after the first predetermined amount of time has elapsed, instructing the user display to provide a second indication.

Abstract: The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.

Abstract: A battery pulsation system including a battery string including a plurality of cells, the battery string defining a positive end and a negative end, a first pulsation device connected to a first cell of the battery string to supply a first pulsation energy thereto, the first cell includes a first positive terminal and a first negative terminal and is generally adjacent to the positive end of the battery string, wherein the first pulsation device applies a greater portion of the first pulsation energy to the first negative terminal than the first positive terminal, and a second pulsation device connected to a second cell of the battery string to supply a second pulsation energy thereto, the second cell includes a second positive terminal and a second negative terminal and is generally adjacent to the negative end of the battery string, wherein the second pulsation device applies a greater portion of the second pulsation energy to the second positive terminal than the second negative terminal.

Abstract: A battery maintenance system including a battery, an electrical pulsation device configured to supply electrical pulsation energy to the battery during at least one pulsation period, a battery charger configured to transfer electrical energy to the battery during at least one charging period and a schedule configured for scheduling operation of the electrical pulsation device and the battery charger such that there is substantially no overlap between the pulsation period and the charging period.

Abstract: A battery pulsation system including a battery string including a plurality of cells, the battery string defining a positive end and a negative end, a first pulsation device connected to a first cell of the battery string to supply a first pulsation energy thereto, the first cell includes a first positive terminal and a first negative terminal and is generally adjacent to the positive end of the battery string, wherein the first pulsation device applies a greater portion of the first pulsation energy to the first negative terminal than the first positive terminal, and a second pulsation device connected to a second cell of the battery string to supply a second pulsation energy thereto, the second cell includes a second positive terminal and a second negative terminal and is generally adjacent to the negative end of the battery string, wherein the second pulsation device applies a greater portion of the second pulsation energy to the second positive terminal than the second negative terminal.

Abstract: A method for cooling a battery after a charging phase including the steps of providing a user display, identifying a starting point at which the charging phase of the battery transitions to a cooling phase, when the starting point is identified, instructing the user display to provide a first indication for a first predetermined amount of time, and after the first predetermined amount of time has elapsed, instructing the user display to provide a second indication.

Abstract: A method of applying pulsation energy to an online battery backup system including the steps of (1) sampling at least one voltage sampling circuit to monitor a float voltage drop across the terminals of each battery unit within a plurality of battery units; (2) selecting from among the plurality of battery units the unit having the greatest float voltage drop; (3) operating a pulse generation circuit to apply pulsation energy across the terminals of only the selected battery unit; and (4) ceasing to operate the pulse generation circuit in response to a predetermined trigger. A generally corresponding method may be performed on each battery cell within the plurality of battery units. Also, battery pulsation systems for an online battery backup system including means for applying pulsation energy across the terminals of only a selected battery unit or a selected battery cell.

Abstract: A method for charging a battery including the steps of providing a battery charger, connecting the battery to the battery charger, supplying a charging current to the battery from the battery charger to increase a charge of the battery, and ceasing to supply the charging current to the battery once the charge of the battery has reached a threshold value, wherein said threshold charge value is selected to correspond with a percentage of a full charge of said battery that occurs prior to substantial gassing of said battery.