Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.

Abstract: Methods of inactivation of a virus in a sample comprising a protein component are provided. Also provided are methods of reducing protein degradation or modification in to the presence of a reactive species, such as a reactive species generated as a result of UV exposure, are also provided. In another aspect, a method of reducing oxidation of methionine residues, tryptophan residues or both methionine and tryptophan residues in a protein subjected to UV light is provided. The disclosed methods can be performed at any scale and can be automated as desired.

Abstract: The invention relates to cell culture media optimized for exposure to ultraviolet C (UVC) light exposure and related methods.

Abstract: Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.

Abstract: A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.

Abstract: A method of handling grey water and optionally at least a portion of the black water for disposal from vehicles such as boats, recreational vehicles or rural dwellings such as cottages, camps and the like, not having direct access to sewer systems, involves the treatment of such waste water with ozone. A batch system is used whereby a predetermined quantity of water to be treated is captured in a treatment tank. A circulating pump withdraws water from the tank and pushes the water along a treatment conduit, the conduit containing an ozone/water mixer and returns the water to the treatment tank. The treatment conduit has a discharge valve that is maintained in a closed position for a selected period of time. Ozone is fed to the ozone/water mixer from an ozone generator which is advantageously fitted with an air pump to pressurize the feed gas.

Abstract: A method of handling grey water and optionally at least a portion of the black water for disposal from vehicles such as boats, recreational vehicles or rural dwellings such as cottages, camps and the like, not having direct access to sewer systems, involves the treatment of such waste water with ozone. A batch system is used whereby a predetermined quantity of water to be treated is captured in a treatment tank. A circulating pump withdraws water from the tank and pushes the water along a treatment conduit, the conduit containing an ozone/water mixer and returns the water to the treatment tank. The treatment conduit has a discharge valve that is maintained in a closed position for a selected period of time. Ozone is fed to the ozone/water mixer from an ozone generator which is advantageously fitted with an air pump to pressurize the feed gas. After the selected treatment time, the batch is discharged to waste through the discharge valve.

Abstract: A method of handling grey water and optionally at least a portion of black water for disposal from vehicles such as boats, recreational vehicles or rural dwellings such as cottages, camps and the like, not having direct access to sewer systems, involves the treatment of such waste water with ozone. A batch system is used whereby a predetermined quantity of water to be treated is captured in a treatment tank. A circulating pump withdraws water from the tank and pushes the water along a treatment conduit, the conduit containing an ozone/water mixer and returns the water to the treatment tank. The treatment conduit has a discharge valve that is maintained in a closed position for a selected period of time. Ozone is fed to the ozone/water mixer from an ozone generator which is advantageously fitted with an air pump to pressurize the feed gas. After the selected treatment time, the batch is discharged to a drain through the discharge valve.

Abstract: A high volume rotary selector valve having a modular structure is disclosed. The disclosed device operates to select one of a plurality of individual source lines of pressurized oil, gas, or other matter to divert the contents of the selected line to a predetermined destination for metering, testing, or sampling without interrupting the flow from other source lines. The modular design permits a large number of input lines to be attached to the valve without a corresponding increase in the valve's diameter or material thickness. Additionally, the modular design affords the user the ability to upgrade an existing selector valve system to increase the number of inlet ports, change the size of the inlet or outlet ports, or change the inlet or outlet port connector types.

Abstract: A composition is provided comprising about 0.1 to 15 mg/ml of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.

Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.

Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.

Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.

Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.

Abstract: A motor control system (10) for controlling a multiple number of motors, each motor having a multiple number of phases. System (10) can be used to dynamically vary the step sizes from microsteps to full steps (i.e. "syncro-stepping the motor").

Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.