Abstract: An improved method and apparatus for handling Drosophila including a simplified nozzle adapter designed to cooperate with the porous cellular obstruction plug in the end of a culture container to permit the passage of carbon dioxide gas therethrough to anesthetize the flies therein. After transferring flies to the sorting chamber for completing a selection process, the fly specimens may be simply and readily transferred to other study containers or culture vials through a suction apparatus positioned in the neck or entrance of the container to receive them.