Patents by Inventor Roger S. Lasken
Roger S. Lasken has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9487823Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a complex nucleic acid sample such as genomic samples using one, a few, or more primers such that, during replication, the replicated strands are displaced from the nucleic acid molecules in the sample by strand displacement replication of another replicated strand. It was discovered that highly complex nucleic acid samples can be efficiently amplified using only one or a few primers having specific nucleic acid sequences. The one or few primers are complementary to nucleic acid sequences distributed throughout nucleic acid in the sample.Type: GrantFiled: December 20, 2002Date of Patent: November 8, 2016Assignee: QIAGEN GMBHInventors: Roger S. Lasken, Michael Egholm, Osama A. Alsmadi
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Publication number: 20080128298Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: ApplicationFiled: October 12, 2007Publication date: June 5, 2008Inventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken
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Patent number: 7297485Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: GrantFiled: May 2, 2003Date of Patent: November 20, 2007Assignee: QIAGEN GmbHInventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken
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Patent number: 7074600Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be ?29 DNA polymerase.Type: GrantFiled: October 15, 2002Date of Patent: July 11, 2006Assignee: Qiagen GmbHInventors: Frank B. Dean, Roger S. Lasken, Linhua Fang, A. Fawad Faruqi, Osama A. Alsmadi, Mark D. Driscoll, Seiyu Hosono, Michele Wisniewski, Wanmin Song
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Patent number: 6977148Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be ?29 DNA polymerase.Type: GrantFiled: October 15, 2001Date of Patent: December 20, 2005Assignee: Qiagen GmbHInventors: Frank B. Dean, Roger S. Lasken, Linhua Fang, A. Fawad Faruqi, Osama A. Alsmadi, Mark D. Driscoll, Seiyu Hosono
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Publication number: 20040161742Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be &phgr;29 DNA polymerase.Type: ApplicationFiled: October 15, 2001Publication date: August 19, 2004Inventors: Frank B. Dean, Roger S. Lasken, Linhua Fang, A. Fawad Faruqi, Osama A. Alsmadi, Mark D. Driscoll, Seiyu Hosono
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Publication number: 20040126764Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a complex nucleic acid sample such as genomic samples using one, a few, or more primers such that, during replication, the replicated strands are displaced from the nucleic acid molecules in the sample by strand displacement replication of another replicated strand. It was discovered that highly complex nucleic acid samples can be efficiently amplified using only one or a few primers having specific nucleic acid sequences. The one or few primers are complementary to nucleic acid sequences distributed throughout nucleic acid in the sample.Type: ApplicationFiled: December 20, 2002Publication date: July 1, 2004Inventors: Roger S. Lasken, Michael Egholm, Osama A. Alsmadi
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Publication number: 20030228613Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: ApplicationFiled: May 2, 2003Publication date: December 11, 2003Inventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken
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Publication number: 20030207267Abstract: Processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences. A kit containing components for use in the invention is also described.Type: ApplicationFiled: July 31, 2001Publication date: November 6, 2003Inventors: Roger S. Lasken, Frank B. Dean, John Nelson
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Patent number: 6617137Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be &phgr;29 DNA polymerase.Type: GrantFiled: October 18, 2001Date of Patent: September 9, 2003Assignee: Molecular Staging Inc.Inventors: Frank B. Dean, Roger S. Lasken
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Publication number: 20030143587Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be &phgr;29 DNA polymerase.Type: ApplicationFiled: October 15, 2002Publication date: July 31, 2003Applicant: Molecular Staging, Inc.Inventors: Frank B. Dean, Roger S. Lasken, Linhua Fang, A. Fawad Faruqi, Osama A. Alsmadi, Mark D. Driscoll, Seiyu Hosono, Michele Wisniewski, Wanmin Song
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Publication number: 20030118998Abstract: Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be &phgr;29 DNA polymerase.Type: ApplicationFiled: October 18, 2001Publication date: June 26, 2003Inventors: Frank B. Dean, Roger S. Lasken
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Patent number: 6323009Abstract: Processes for the amplification of target DNA sequences in the form of single or double stranded circular DNA molecules, especially those present in colony and plaque extracts, using multiple specific and/or random sequence oligonucleotide primers are disclosed along with methods for detecting such amplified target sequences. A kit containing components for use in the invention is also described.Type: GrantFiled: June 28, 2000Date of Patent: November 27, 2001Assignee: Molecular Staging, Inc.Inventors: Roger S. Lasken, Frank B. Dean, John Nelson
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Patent number: H1531Abstract: The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.Type: GrantFiled: April 18, 1994Date of Patent: May 7, 1996Inventors: Ilse I. Blumentals, Roger S. Lasken, Brian J. Schmidt, Mary C. Longo, A. John Hughes, Jr., Deb K. Chatterjee