Abstract: An example of an apparatus to detect a target component in a test sample is provided. The apparatus includes a sample pad to receive the test sample. The apparatus further includes a conjugate pad in communication with the sample pad to receive the test sample. The test sample is to interact with a first luminescent particle having a protein and a plurality of primary antibodies to form a conjugated sample. A primary antibody binds with the target component. In addition, the apparatus includes an enhancement pad in communication with the conjugate pad to receive the conjugated sample. The conjugated sample is to interact with a second luminescent particle having a chemical molecule which links with the protein to form an enhanced sample. Furthermore, the apparatus includes a membrane in communication with the enhancement pad to receive the enhanced sample. The membrane includes a test region treated with the target component.