Abstract: Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

Abstract: Herein is reported a method for determining the concentration of a therapeutic antibody in a tissue of an experimental animal to whom the therapeutic antibody had been administered, wherein the interference from residual blood in a tissue sample of the experimental animal, which is used for determining the concentration of the therapeutic antibody in said tissue, is reduced by applying an inert reference antibody that does not penetrate into said tissue, whereby the inert reference antibody is administered 2 to 10 minutes prior to obtaining the tissue and blood sample.

Abstract: Signal processing is used to detect transient signals in the presence of noise. Two embodiments are disclosed. In both embodiments, the time series from a remote sensor is broken into a number of short time series. The power spectrum of each short time series are then calculated along with the mean noise level. The moments of each peak in every power spectrum are calculated and the peak with the largest power selected from each power spectrum. A histogram of the moments from these selected peaks is generated and normalized to become a measured PDF. In addition, a pre-determined PDF is derived, in the same method as above, from theoretically calculated noise, numerically simulated noise, or measured noise. Comparison between the measured and pre-determined PDF's establish the detection of a transient signal. The first embodiment compares the area between the measured and pre-determined PDF's against a threshold to determine detection.

Abstract: Composite materials are provided which include one or more felt layers, impregnated, in a compressed state, with a reinforcing polymeric resin, e.g., a water-borne phenolic resin. The composite material may further include other layers, e.g., a fibrous layer such as glass cloth or scrim.