Abstract: Methods for introducing recombinant adeno-associated virus (rAAV) virions into a cell or cells of a secretory gland are described. Recombinant AAV virions containing a heterologous gene are introduced into a duct of a secretory gland resulting in transduction of one or more secretory gland cells. Once a secretory gland cell is transduced by the rAAV virion, the heterologous gene is expressed and the expression product is then secreted. Exemplary examples of secretory glands are the liver, the submandibular gland, the parotid gland, and the sublingual gland. Using the methods of the invention, therapeutic levels of a protein are achieved. Methods for treating hemophilia are also disclosed.