Abstract: Disclosed are W-position modified cytidine nucleotides of formula (I). Provided herein are methods of chemical synthesis of AP-modified cytidine nucleoside triphosphates and their applications as well as uses of the cytidine analogues for the synthesis of modified nucleic acids. The nucleic acid molecule includes DNA, RNA or a combination of DNA/RNA. One of many applications of modified cytidine nucleotides described herein is enzyme selection, when an enzyme of interest bears an activity of an esterase, amidase, oxidoreductase, lyase, ligase or other enzymatic activity, formula (I) wherein the substituants are as defined in the appended claims.

Abstract: The invention generally relates to the field of protein sequences and of generation of functional protein sequences. More particularly, the invention concerns a method for generating functional protein sequences with generative adversarial networks. The described method for functional sequence generation comprises plurality of steps, each of which is crucial to ensure the high percentage of functional sequences in the final sequence set: selecting a plurality of existing protein sequences to define the approximate sequence space for the later generated synthetic sequences, processing the selected protein sequences, approximating the unknown true distribution of amino acids of the pre-processed sequences using a variation of generative adversarial networks, obtaining protein sequences from the approximated distribution, processing of the obtained protein sequences. The described method provides a resource (e.g.

Abstract: The present invention relates to catalytic biomolecule characterization and microfluidics. It is used for identification of nucleic acids encoding active catalytic molecules in the plurality of nucleic acids and for gathering information about catalytic biomolecule activity. It can also be used for exploring different properties of regulating sequences that modulate expression of catalytic biomolecules by recording that information into the DNA sequence of the same catalytic biomolecule using microfluidic techniques.

Abstract: Disclosed are W-position modified cytidine nucleotides of formula (I). Provided herein are methods of chemical synthesis of AP-modified cytidine nucleoside triphosphates and their applications as well as uses of the cytidine analogues for the synthesis of modified nucleic acids. The nucleic acid molecule includes DNA, RNA or a combination of DNA/RNA. One of many applications of modified cytidine nucleotides described herein is enzyme selection, when an enzyme of interest bears an activity of an esterase, amidase, oxidoreductase, lyase, ligase or other enzymatic activity, formula (I) wherein the substituants are as defined in the appended claims.

Abstract: The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof.

Abstract: The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof.

Abstract: The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and to a process for their preparation by overexpression in suitable microbial expression systems.

Abstract: The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and to a process for their preparation by overexpression in suitable microbial expression systems.

Abstract: The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and to a process for their preparation by overexpression in suitable microbial expression systems.