Abstract: Compositions comprising glycosyl hydrolase enzymes are provided, as are methods for their use to depolymerize hemicellulose, cellulose, lignin and pectin in biomass in order to produce products such as simple sugars. The enzymes, isolated from Aspergillus nidulans and Phanerochaete chrysosporium, were characterized, and synergistic mixtures of the enzymes were produced and used to generate simple sugars from biomass without the need to pretreat the biomass before digestion. The enzyme blends generally comprise two or more enzymes, which may be from the same fungus or from two different fungi, and are used for efficient and cost effective complete degradation of lignocelluloses. Applications of this technology include biofuel production.

Abstract: Compositions comprising glycosyl hydrolase enzymes are provided, as are methods for their use to depolymerize hemicellulose, cellulose, lignin and pectin in biomass in order to produce products such as simple sugars. The enzymes, isolated from Aspergillus nidulans and Phanerochaete chrysosporium, were characterized, and synergistic mixtures of the enzymes were produced and used to generate simple sugars from biomass without the need to pretreat the biomass before digestion. The enzyme blends generally comprise two or more enzymes, which may be from the same fungus or from two different fungi, and are used for efficient and cost effective complete degradation of lignocelluloses. Applications of this technology include biofuel production.

Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.

Abstract: Methods of continuous production of a product of interest using a filamentous fungus are provided. The fungus is auxotrophic for a nutrient that is required for fungal growth, but not for fungal synthesis of the product of interest. Under growth limiting culture conditions, in which the nutrient is absent from the culture medium, the amount of fungus remains constant or nearly constant and nutrients are not used up for growth but instead are available for synthetic pathways which produce the product of interest.

Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.

Abstract: Hemicellulase (xylanase) enzymes possessing endo-xylanase, laminarase, mannanase, arabinase and arabinofuranosidase activity are useful to degrade hemicellulose and other substrates to their constituent sugars.

Abstract: Thermostable cellulase enzyme systems comprising at least one each of a thermostable endoglucanase, an exo-processive-endoglucanase, and a ?-glucosidase carry out the complete, coordinated hydrolysis of crystalline cellulose to monomeric glucose.