Patents by Inventor Rolf-Günther Werner

Rolf-Günther Werner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for large scale production of recombinant DNA-derived tPA or K2S molecules
    Patent number: 7462476
    Abstract: The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant thereof or a (Kringle 2 Serine) K2S molecule or a variant thereof in prokaryotic cells, wherein the tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by the method. The invention further relates to the DNA molecules and the use of the DNA molecules in the methods.
    Type: Grant
    Filed: May 23, 2005
    Date of Patent: December 9, 2008
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Methods for large scale protein production in prokaryotes
    Patent number: 7087412
    Abstract: The invention belongs to the field of protein production in prokaryotic cells. The invention relates to methods for the production of recombinant DNA-derived heterologous protein in prokaryotic cells, wherein the heterologous protein is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the heterologous protein operably linked to the DNA coding for the signal peptide OmpA.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: August 8, 2006
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Method for large scale production of recombinant DNA-derived TPA or K2S molecules
    Patent number: 6955910
    Abstract: The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant therof or a (Kringle 2 Serine) K2S molecule or a variant therof in prokaryotic cells, wherein the tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by the method. The invention further relates to the DNA molecules and the use of the DNA molecules in the methods.
    Type: Grant
    Filed: November 14, 2001
    Date of Patent: October 18, 2005
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Methods for large scale productions of recombinant DNA-derived tPA or K2S molecules
    Publication number: 20050202040
    Abstract: The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant therof or a (Kringle 2 Serine) K2S molecule or a variant therof in prokaryotic cells, wherein the tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by the method. The invention further relates to the DNA molecules and the use of the DNA molecules in the methods.
    Type: Application
    Filed: May 23, 2005
    Publication date: September 15, 2005
    Applicant: Boehringer Ingelheim International GmbH
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Preparation of a recombinant protein in a prokaryotic host cell
    Patent number: 6821755
    Abstract: The invention relates to advantageous processes for preparing heterologous proteins in prokaryotic host cells by improved codon use and/or expression of tRNAs which code for codons occurring rarely in said host cell.
    Type: Grant
    Filed: July 27, 2001
    Date of Patent: November 23, 2004
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Rolf-Günther Werner, Klaus Bergemann, Friedrich Götz, Andreas Peschel
  • Methods for large scale production of recombinant DNA-Derived TPA or K2S molecules
    Publication number: 20030049729
    Abstract: The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant therof or a (Kringle 2 Serine) K2S molecule or a variant therof in prokaryotic cells, wherein the tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by the method. The invention further relates to the DNA molecules and the use of the DNA molecules in the methods.
    Type: Application
    Filed: November 14, 2001
    Publication date: March 13, 2003
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner
  • Methods for large scale protein production in prokaryotes
    Publication number: 20030013150
    Abstract: The invention belongs to the field of protein production in prokaryotic cells. The invention relates to methods for the production of recombinant DNA-derived heterologous protein in prokaryotic cells, wherein the heterologous protein is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for the heterologous protein operably linked to the DNA coding for the signal peptide OmpA.
    Type: Application
    Filed: November 14, 2001
    Publication date: January 16, 2003
    Inventors: Jiradej Manosroi, Aranya Manosroi, Chatchai Tayapiwatana, Friedrich Goetz, Rolf-Guenther Werner