Abstract: A disposable container comprising a side wall, top and bottom, wherein the side wall comprises a first flexible material and the bottom comprises a rigid or semi-rigid material. The side wall top and bottom are joined together to define the container with an interior compartment for keeping a fluid inside the container. The bottom comprises at least one opening that is fluid-tightly covered with an assembly comprising a second flexible material. The assembly comprises at least one port which provides access to the interior compartment of the container. The invention also relates to a mixing system comprising the disposable container.

Abstract: A container comprising a first material and a second material, the first material being a flexible material and the second material being a rigid or semi-rigid material and at least partially defining an interior compartment for keeping a fluid inside the container characterized in that the flexible material and/or the semi-rigid or rigid material have thermoplastic properties and wherein the flexible material and the semi-rigid or rigid material are joined together by a seal or joint which comprises a heat seal and an adhesive seal.

Abstract: A disposable container comprising a side wall, top and bottom, wherein the side wall comprises a first flexible material and the bottom comprises a rigid or semi-rigid material. The side wall top and bottom are joined together to define the container with an interior compartment for keeping a fluid inside the container. The bottom comprises at least one opening that is fluid-tightly covered with an assembly comprising a second flexible material. The assembly comprises at least one port which provides access to the interior compartment of the container. The invention also relates to a mixing system comprising the disposable container.

Abstract: A container comprising a first material and a second material, the first material being a flexible material and the second material being a rigid or semi-rigid material and at least partially defining an interior compartment for keeping a fluid inside the container characterized in that the flexible material and/or the semi-rigid or rigid material have thermoplastic properties and wherein the flexible material and the semi-rigid or rigid material are joined together by means of a seal or joint which comprises a heat seal and an adhesive seal

Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.

Abstract: The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles.

Abstract: The present invention relates to methods of isolating biomolecules. More particularly, the invention relates to methods for isolating antibodies (mAbs) and related proteins including antibody fragments (Fabs) under conditions where they are positive and relatively hydrophobic and will react with negatively charged polymer to form polymer-protein complexes which precipitate. The isolation can be accomplished using inexpensive and biocompatible negatively charged polymers such as polyacrylic acid or carboxymethyldextran polymers of various molecular weights as precipitant. It occurs at relatively high concentrations of polymer (e.g. 10%) and high salt concentration (>50 mM) and conductivity (e.g. >10 mS/cm) over wide range of pH.

Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.

Abstract: A tank adapted to be used for suspending separation media slurry before transferring the separation media slurry to a column or a container comprises: an impeller (7) adapted to rotate in order to mix the media slurry, a nozzle (9) provided protruding into the tank from the bottom (8) thereof, and an adjustable pipe (5) having an open end (6) positioned in the tank and which can be adjusted in position. The nozzle (9) and the adjustable pipe (5) are adapted to be connected to a pump (3) adapted to pump liquid from the adjustable pipe (5) when the open end (6) of the adjustable pipe (5) has been positioned in an uppermost liquid phase provided in the tank when the media slurry has started to sediment and pump the liquid, possibly containing a small amount of media, through the nozzle (9) back into the tank from the tank bottom (8) in order to start re-suspending media that has sedimented in the tank.

Abstract: The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles.

Abstract: The present invention relates to a process of isolating one or more target compounds, wherein the clarification of feed is performed using partitioning in a multiphase system comprising a first polymer, which is a synthetic poly(acid), a second synthetic polymer, which is a poly(ether), and at least one salt, which clarification is followed by at least one step of affinity chromatography. The molecular weight of the poly(acid) may be in the range of 1000-100,000 Da. The target compound is preferably a biomolecule, such as a monoclonal antibody.

Abstract: A tank adapted to be used for suspending separation media slurry before transferring the separation media slurry to a column or a container comprises: an impeller (7) adapted to rotate in order to mix the media slurry, a nozzle (9) provided protruding into the tank from the bottom (8) thereof, and an adjustable pipe (5) having an open end (6) positioned in the tank and which can be adjusted in position. The nozzle (9) and the adjustable pipe (5) are adapted to be connected to a pump (3) adapted to pump liquid from the adjustable pipe (5) when the open end (6) of the adjustable pipe (5) has been positioned in an uppermost liquid phase provided in the tank when the media slurry has started to sediment and pump the liquid, possibly containing a small amount of media, through the nozzle (9) back into the tank from the tank bottom (8) in order to start re-suspending media that has sedimented in the tank.