Abstract: The present invention provides a method for preparing a modular scaffold that can bind to a target antigen and a method for engineering a bispecific functional agent consisting of an existing polypeptide binder fused at its C-terminus with said modular scaffold.

Abstract: The present invention provides a triple-mode antibody display system that simultaneously matures, displays and secretes an antibody to a target of interest. An antibody in vivo-matured and complexed with membrane anchored bait can be expressed on the surface of the host cell, while complexed with a soluble bait the antibody is secreted from the host cell. Methods of using the system for identifying binders that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the protein binder display system are also provided along with methods of use thereof.

Abstract: The present invention provides a triple-mode antibody display system that simultaneously matures, displays and secretes an antibody to a target of interest. An antibody in vivo-matured and complexed with membrane anchored bait can be expressed on the surface of the host cell, while complexed with a soluble bait the antibody is secreted from the host cell. Methods of using the system for identifying binders that bind specifically to an antigen of interest are also provided.

Abstract: The present invention provides a method for preparing a modular scaffold that can bind to a target antigen and a method for engineering a bispecific functional agent consisting of an existing polypeptide binder fused at its C-terminus with said modular scaffold.

Abstract: Provided, among other things, is a yeast cell comprising: (A) a recombinant DNA that constitutively or inducibly expresses a cytidine deaminase comprising sequence with about 90% sequence identity or more with a cytidine deaminase domain of (i) SEQ ID NO. 2 or SEQ ID NO. 4, or (ii) a chimera between the two starting with SEQ ID NO. 3 or SEQ ID NO. 4 sequence and having one transition to end in SEQ ID NO. 1 or SEQ ID NO. 2 sequence, or (iii) a chimera between the two starting with SEQ ID NO. 1 or SEQ ID NO. 2 sequence and having one transition to end in SEQ ID NO. 3 or SEQ ID NO. 4 sequence; and (B) a second recombinant DNA that constitutively or inducibly expresses a binding scaffold protein for presentation on the outer surface of the yeast, wherein the cytidine deaminase as expressed by the first recombinant DNA is effective to contribute to a mutagenic process for inducing mutations in the binding scaffold protein of the yeast cell.