Abstract: A method and system for enabling the determination of kinetic rates of reaction within a fluid of interest including directing fluid flow exiting a test bed to a multi-port switching valve. The multi-port switching valve switches the fluid to a number of channels connected to a number of interchangeable in-flow extraction cartridges. Analytes of interest from the fluid flow are captured on an extraction medium to accumulate over time. Rates are determined by (i) sequentially channeling the fluid through each of the plurality of flow paths for a preselected time duration, (ii) analyzing the extraction cartridges, and computing the kinetic rate of reaction.

Abstract: A method for installing an in situ, low-permeability temporary horizontal barrier at depth for vertical containment of a soil mass including installing a low permeability vertical barrier having walls located to contain a predetermined soil volume, the vertical barrier having a horizontal surface area in a plane perpendicular to its walls; and injecting low permeability reagents capable of forming a temporary horizontal barrier in a pattern suitable for creating a horizontal subsurface barrier joined to all of the vertical barrier walls to form an in situ barrier.

Abstract: A pH treatment and method for extraction of leachable resources and pollutants from sludge. A volume of wastewater sludge is initially dewatered followed by adding pH adjusting agents to the dewatered sludge to remove acid/base-soluble contaminants. The acid/base-soluble contaminants are flushed out or dewatered from the treated product. Treated sludge can then be sent to storage and held for beneficial uses.

Abstract: A method for installing an in situ, low-permeability temporary horizontal barrier at depth for vertical containment of a soil mass including installing a low permeability vertical barrier having walls located to contain a predetermined soil volume, the vertical barrier having a horizontal surface area in a plane perpendicular to its walls; and injecting low permeability reagents capable of forming a temporary horizontal barrier in a pattern suitable for creating a horizontal subsurface barrier joined to all of the vertical barrier walls to form an in situ barrier.

Abstract: A method and system for enabling the determination of kinetic rates within a fluid of interest including directing fluid flow exiting a test bed to a multi-port switching valve. The multi-port switching valve to switches the fluid to a number of channels connected to in in-flow extraction cartridge. Analytes of interest from the fluid flow our concentrated and captured on an extraction medium. Rates are determined by (i) sequentially channeling the fluid through the each of the plurality of channels for a preselected time duration, (ii) analyzing the extraction cartridges, and computing the kinetic rate of interest.

Abstract: Contaminant mass collection in saturated sedimentary environments for bioavailability determination. A casing includes a screen between the environment that is subject to sampling, such as a saturated sediment and the device itself. The casing includes a water intake zone, a pump, and sorptive media. The water intake zone, the pump, the screen and the sorptive media, are all operably linked in sequence. The screened casing is secured to form an in situ device; the screen is in fluid communication with the water intake zone and excludes endemic sediments and aquatic life. The in situ device is deployed in the saturated sedimentary environment. The pump operates to concentrate analytes from the selected environment in the sorptive media, where the concentrated analytes include the analyte mass of time-weighted fluid samples.

Abstract: A pH treatment and method for extraction of leachable resources and pollutants from sludge. A volume of wastewater sludge is initially dewatered followed by adding pH adjusting agents to the dewatered sludge to remove acid/base-soluble contaminants. The acid/base-soluble contaminants are flushed out or dewatered from the treated product. Treated sludge can then be sent to storage and held for beneficial uses.

Abstract: A monitoring assembly (201) with an intake (213) has at least one pump (210) featuring at least one pump channel mounted in the monitoring assembly (201). A plurality of fluid lines are coupled to the at least one pump (210). At least one filter cartridge (315) is also mounted in the assembly. Each filter cartridge (315) is separately coupled by one of the plurality of fluid lines to one of the pump channels, where each filter cartridge (315) contains material for extracting an analyte, and where the at least one pump operates to separately push fluid through the at least one filter cartridge (315). The filter cartridge (315) operates to separate fluid into constituent parts.

Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.

Abstract: A method for expression of engineered constructs containing sequences coding for functional genes of interest in E. coli strains. Here tceA and tceB are fused to an inducible, active promoter to optimize transcription of the open reading frames, and an introduced consensus Shine-Dalgarno sequence allowing for optimal ribosome binding and translation of the open reading frames, and codons optimized for expression in E. coil. This over-expressed protein is then used to design appropriate mass spectro metric methods for environmental detection.

Abstract: An amino acid chain of interest is selected or captured for amplification. The amino acid chain of interest including at least one amino acid sequence is denatured and read. The read amino acid sequence is transcribed to synthesize a mRNA equivalent. The amino acid chain of interest is then amplified using a biological system.

Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.

Abstract: Contaminant mass collection in saturated sedimentary environments for bioavailability determination. A casing includes a screen between the environment that is subject to sampling, such as a saturated sediment and the device itself. The casing includes a water intake zone, a pump, and sorptive media. The water intake zone, the pump, the screen and the sorptive media, are all operably linked in sequence. The screened casing is secured to form an in situ device; the screen is in fluid communication with the water intake zone and excludes endemic sediments and aquatic life. The in situ device is deployed in the saturated sedimentary environment. The pump operates to concentrate analytes from the selected environment in the sorptive media, where the concentrated analytes include the analyte mass of time-weighted fluid samples.

Abstract: A method for in situ monitoring within a specified environment. The method includes locating a housing in a well, wherein a set of pumps and a plurality of test beds are inserted. Each of the set of pumps are controlled by signals from the control system to push water from each pump into one of the plurality of separate test beds where, after flowing through each of the test beds, effluent flows into an effluent storage device.

Abstract: Methods and systems for dehalogenating organohalides are disclosed. In one respect, the systems can generate hydrogen in an electrolysis cell and supply the hydrogen to anaerobic dehalogenating bacteria to decontaminate organohalides at a contamination site.

Abstract: A method for in situ monitoring within a specified environment. The method includes locating a housing in a well, wherein a set of pumps and a plurality of test beds are inserted. Each of the set of pumps are controlled by signals from the control system to push water from each pump into one of the plurality of separate test beds where, after flowing through each of the test beds, effluent flows into an effluent storage device.

Abstract: A monitoring assembly (201) with an intake (213) has at least one pump (210) featuring at least one pump channel mounted in the monitoring assembly (201). A plurality of fluid lines are coupled to the at least one pump (210). At least one filter cartridge (315) is also mounted in the assembly. Each filter cartridge (315) is separately coupled by one of the plurality of fluid lines to one of the pump channels, where each filter cartridge (315) contains material for extracting an analyte, and where the at least one pump operates to separately push fluid through the at least one filter cartridge (315). The filter cartridge (315) operates to separate fluid into constituent parts.

Abstract: A method for environmental monitoring and bioprospecting includes the steps of: (a) utilizing a testing device having: (i) a container having a fluid inlet and outlet, (ii) a plurality of capillary microcosms situated within the container, each of these capillaries having an inlet and outlet that are configured so as to allow for fluid flow through the capillaries, each of these capillaries further having a means for covering its inlet and outlet so as to prevent flow through the capillary, (iii) a pump connected to the container outlet, the pump being configured so as to draw fluid from the surrounding environment into the container's inlet and through the capillaries, (iv) connected to the outlet of the container, a means for collecting the flow through the container, and (v) a check valve connected downstream of the container to prevent the backflow of fluid into the container, (b) adding specified test substances to the device's capillaries, wherein these substances are to be analyzed for their ability to

Abstract: The invention relates to a consortium of microorganisms that can be used to dehalogenate a chemical composition. Methods of use of the same for biomass production and for use of the same in bioremediation are described.

Abstract: A system for in situ monitoring within a specified environment includes a housing with an intake inserted into the environment. A plurality of pumps are included in the housing with a number of test beds, each being separately coupled to one of the number of pumps, where each of the number of test beds holds material and where each of the plurality of pumps operate to separately push fluid through a coupled test bed. An effluent storage device is disposed to receive effluent from the number of test beds.