Abstract: Ice crystal growth inhibiting agents and process for preparing an extract of these agents in which Zoarces viviparus blood is extracted, it is cooled, the supernatant constituting the Zoarces viviparus serum containing the ice crystal growth inhibiting agents is collected, and then the supernatant is frozen. The present invention also relates to a process for using these agents for the manufacture of a food product.

Abstract: The freezing point of a food product subjected to freezing temperatures is increased by adding to the food product a juice obtained from berries of sea buckthorn or an aqueous extract obtained from sea buckthorn berry or leaf tissue. The food product containing the added sea buckthorn substance is then cooled to freeze the product.

Abstract: Sea buckthorn berry juice and aqueous extracts obtained from sea buckthorn berry and leaf tissue provide an ice nucleating agent and thereby operate, upon addition of the juice and/or extract to a freezeable food product, to increase the freezing temperature of the food product. A protein and a protein-lipid aggregate having the ice-nucleating activity may be isolated from the juice and/or extract. The extracts may be obtained by extracting the tissue with an aqueous solution containing a saccharide, particularly pectin alone or in combination with another saccharide.