Abstract: Embodiments of the present disclosure include materials and methods relating to sterilization devices. In certain embodiments, materials and methods disclosed herein relate to pre-surgical sterilization of an appendage of a subject using a device that reduces surgical preparation time and microbial growth. Certain embodiments of the present disclosure disclose materials and methods to ameliorate high costs associated with medical care, especially the utilization of costly elective hospital services, by for example reducing operating room costs and increasing efficiency without compromising patient health.