Patents by Inventor Roman Rozhkov
Roman Rozhkov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230175046Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: ApplicationFiled: February 1, 2023Publication date: June 8, 2023Inventors: David LIGHT, Wolfgang HINZ, Ronald CICERO, Christina INMAN, Paul KENNEY, Alexander MASTROIANNI, Roman ROZHKOV, Yufang WANG, Jeremy GRAY, Marc GLAZER, Dmitriy GREMYACHINSKIY
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Patent number: 11578356Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.Type: GrantFiled: July 24, 2020Date of Patent: February 14, 2023Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: David Light, Wolfgang Hinz, Ronald Cicero, Christina Inman, Paul Kenney, Alexander Mastroianni, Roman Rozhkov, Yufang Wang, Jeremy Gray, Marc Glazer, Dmitriy Gremyachinskiy
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Patent number: 11015220Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: GrantFiled: September 6, 2018Date of Patent: May 25, 2021Assignee: Life Technologies CorporationInventors: Theo Nikiforov, Daniel Mazur, Xinzhan Peng, Tommie Lloyd Lincecum, Yuri Belosludtsev, Howard Reese, Dmitriy Gremyachinskiy, Roman Rozhkov, John Mauro, Joseph Beechem, Eric Tulsky, Imad Naasani, Kari Haley, Joseph Treadway
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Publication number: 20200354780Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: ApplicationFiled: July 24, 2020Publication date: November 12, 2020Inventors: David LIGHT, Wolfgang HINZ, Ronald CICERO, Christina INMAN, Paul KENNEY, Alexander MASTROIANNI, Roman ROZHKOV, Yufang WANG, Jeremy GRAY, Marc GLAZER, Dmitriy GREMYACHINSKIY
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Patent number: 10738353Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: GrantFiled: August 31, 2018Date of Patent: August 11, 2020Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: David Light, Wolfgang Hinz, Ronald Cicero, Christina Inman, Paul Kenney, Alexander Mastroianni, Roman Rozhkov, Yufang Wang, Jeremy Gray, Marc Glazer, Dmitriy Gremyachinskiy
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Patent number: 10487357Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.Type: GrantFiled: March 18, 2016Date of Patent: November 26, 2019Assignees: Life Technologies Corporation, Life Technologies GmbHInventors: Wolfgang Hinz, Steven Menchen, Ronald Graham, Peter Vander Horn, Earl Hubbell, Christian Woehler, Roman Rozhkov, Barnett Rosenblum
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Publication number: 20190062830Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: ApplicationFiled: September 6, 2018Publication date: February 28, 2019Inventors: Theo NIKIFOROV, Daniel MAZUR, Xinzhan PENG, Tommie Lloyd LINCECUM, Yuri BELOSLUDTSEV, Howard REESE, Dmitriy GREMYACHINSKIY, Roman ROZHKOV, John MAURO, Joseph BEECHEM, Eric TULSKY, Imad NAASANI, Kari HALEY, Joseph TREADWAY
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Publication number: 20190017107Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: ApplicationFiled: August 31, 2018Publication date: January 17, 2019Inventors: David LIGHT, Wolfgang HINZ, Ronald CICERO, Christina INMAN, Paul KENNEY, Alexander MASTROIANNI, Roman ROZHKOV, Yufang WANG, Jeremy GRAY, Marc GLAZER, Dmitriy GREMYACHINSKIY
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Patent number: 10093972Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: GrantFiled: May 27, 2016Date of Patent: October 9, 2018Assignee: Life Technologies CorporationInventors: Theo Nikiforov, Daniel Mazur, Xinzhan Peng, Tommie Lloyd Lincecum, Yuri Belosludtsev, Howard Reese, Dmitriy Gremyachinskiy, Roman Rozhkov, John Mauro, Joseph Beechem, Eric Tulsky, Imad Naasani, Kari Haley, Joseph Treadway
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Patent number: 10066260Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: GrantFiled: March 13, 2014Date of Patent: September 4, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: David Light, Wolfgang Hinz, Ronald L. Cicero, Christina E. Inman, Paul M. Kenney, Alexander Mastroianni, Roman Rozhkov, Yufang Wang, Jeremy Gray, Marc Glazer, Dmitriy Gremyachinskiy
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Publication number: 20180119217Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.Type: ApplicationFiled: March 18, 2016Publication date: May 3, 2018Applicants: Life Technologies Corporation, Life Technologies GmbHInventors: Wolfgang HINZ, Steven MENCHEN, Ronald GRAHAM, Peter VANDER HORN, Earl HUBBELL, Christian WOEHLER, Roman ROZHKOV, Barnett ROSENBLUM
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Patent number: 9476885Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.Type: GrantFiled: January 6, 2015Date of Patent: October 25, 2016Assignee: Life Technologies CorporationInventors: Eric Tulsky, Kari Haley, Imad Naasani, John Mauro, Roman Rozhkov, Joseph Treadway
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Publication number: 20160265044Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: ApplicationFiled: May 27, 2016Publication date: September 15, 2016Inventors: Theo NIKIFOROV, Daniel MAZUR, Xinzhan PENG, Tommie Lloyd LINCECUM, Yuri BELOSLUDTSEV, Howard REESE, Dmitriy GREMYACHINSKIY, Roman ROZHKOV, John MAURO, Joseph BEECHEM, Eric TULSKY, Imad NAASANI, Kari HALEY, Joseph TREADWAY
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Patent number: 9365838Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: GrantFiled: November 22, 2013Date of Patent: June 14, 2016Assignee: Life Technologies CorporationInventors: Theo Nikiforov, Daniel Mazur, Xinzhan Peng, Tommie Lloyd Lincecum, Yuri Belosludtsev, Howard Reese, Dmitriy Gremyachinskiy, Roman Rozhkov, John Mauro, Joseph Beechem, Eric Tulsky, Imad Naasani, Kari Haley, Joseph Treadway
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Publication number: 20160032371Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.Type: ApplicationFiled: March 13, 2014Publication date: February 4, 2016Inventors: David LIGHT, Wolfgang HINZ, Ronald L. CICERO, Christina E. INMAN, Paul M. KENNEY, Alexander MASTROIANNI, Roman ROZHKOV, Yufang WANG, Jeremy GRAY, Marc GLAZER, Dmitriy GREMYACHINSKIY
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Patent number: 9174187Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.Type: GrantFiled: August 6, 2009Date of Patent: November 3, 2015Assignee: Life Technologies CorporationInventors: Eric Tulsky, Kari Haley, Imad Naasani, John Mauro, Roman Rozhkov, Joseph A. Treadway
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Publication number: 20150233936Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.Type: ApplicationFiled: January 6, 2015Publication date: August 20, 2015Inventors: Eric TULSKY, Kari Haley, Imad Naasani, John Mauro, Roman Rozhkov, Joseph Treadway
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Patent number: 8901333Abstract: Coated fluorescent semiconductor nanoparticles having an organic surface layer of multi-functional surface ligands that include a nanocrystal binding center and one or more covalently attached functional groups or reactive functional groups are described as well as water-dispersible nanoparticles having an organic surface layer or multi-functional surface ligands and methods for the preparation and use of such coated nanoparticles.Type: GrantFiled: September 30, 2009Date of Patent: December 2, 2014Assignee: Life Technologies CorporationInventors: Eric Tulsky, Eric Welch, Bruce Branchaud, Roman Rozhkov
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Publication number: 20140308730Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: ApplicationFiled: November 22, 2013Publication date: October 16, 2014Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Theo NIKIFOROV, Daniel MAZUR, Xinzhan PENG, Tommie Lloyd LINCECUM, Yuri BELOSLUDTSEV, Howard REESE, Dmitriy GREMYACHINSKIY, Roman ROZHKOV, John MAURO, Joseph BEECHEM, Eric TULSKY, Imad NAASANI, Kari HALEY, Joseph TREADWAY
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Patent number: 8603792Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.Type: GrantFiled: March 26, 2010Date of Patent: December 10, 2013Assignee: Life Technologies CorporationInventors: Theo Nikiforov, Daniel Mazur, Xinzhan Peng, Tommie Lloyd Lincecum, Jr., Yuri Belosludtsev, Howard Reese, Dmitriy Gremyachinskiy, Roman Rozhkov, John M. Mauro, Joseph Beechem, Eric Tulsky, Imad Naasani, Kari Haley, Joseph A. Treadway