Abstract: Disclosed herein are method for labeling analytes using spatial barcodes, illumination, and/or photolabile cross-linkers. The methods may generate a set of unique spatial barcodes using limited sets differential nucleotide sequences. The labeling may identify a spatial location of an analyte. Also provided herein are compositions, kits, or system for carrying out the methods.

Abstract: Provided herein are methods, systems, compositions, and kits that can determine spatial information between a plurality of analytes in a sample by tagging such analytes with tags, where identities and/or locations of such tags are previously unknown prior to tagging. Each of a plurality of analyte sequences may be tagged with multiple spatial tags, such that each analyte sequence is associated with a set of two or more spatial tags. The sets of spatial tags may be analyzed to generate a map of analyte sequences. The map may comprise information about the respective absolute positions of each of a set of sequences with respect to a reference sequence. The map may comprise information about the respective probability cloud (or likely location) of each of a set of sequences with respect to a reference sequence.

Abstract: Despite the advance of screening technology, omic-based studies with spatial resolution still requires laborious efforts, hampering the analysis of biology and disease. The present disclosure provides methods, systems, reagents, and platforms to increase the throughput of analyte screening with spatial resolution.

Abstract: Methods, systems, and computer storage mediums including a computer program product for managing data in a computing network are provided. One method includes registering a plurality of buffers with a work queue in a server and assigning ownership to each of the plurality of buffers to a different working thread in a plurality of working threads. The method further includes continuously polling, by a polling thread, the work queue to determine when work requests are received by the work queue, upon receipt of each work request, determining which buffer among the plurality of buffers each work request is associated, and performing each work request on each respective associated buffer by a working thread among the plurality of working threads that owns each respective associated buffer. One system includes a processor for performing the above method and one computer storage medium includes computer code for performing the above method.