Abstract: A mounting device mounts a mounting foot to another member, such as a base pan. The mounting device includes a grommet, a splined washer and a fastener. The grommet has an axially extending post for receiving a mounting foot. The splined washer is configured to lock into the mounting foot and the post. The splined washer has an inner bore for receiving the post. The fastener is received on the post and maintains the splined washer and the mounting foot on the post.

Abstract: A heat exchanger includes a first manifold, a second manifold and a plurality of tubes extending between the first manifold and the second manifold. An adapter is mounted to the first manifold, and the adapter includes a plurality of engagement features engageable by a forming machine to form the heat exchanger into a shape.

Abstract: A drip tube has a generally horizontal section, a generally vertical section and a drip loop connecting the sections. The drip loop is positioned so that its exterior surface is lower than the exterior surfaces of the generally horizontal section and the generally vertical section at the points where they meet the drip loop to provide a location where water may drip.

Abstract: A multi-well cassette configuration and an instrument capable of accepting the cassette and thereafter pre-concentrating and purifying analytes from biological samples held in the cassette wells, such as human serum, for subsequent analysis by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS).

Abstract: Disclosed herein is a waterproof case for protecting electronic devices. The waterproof case includes a flexible waterproof bag attached to a rigid lid that can be opened to allow electronic devices to pass into the bag. When closed, the lid forms a watertight seal that protects the inside of the bag from ingress of water. The lid can include a pass-through jack which allows a user to connect an electronic device, such as an audio device, to an output through the lid. The case may also be incorporated into a flexible attachment system, such as an armband or swimbelt. Methods of connecting an electronic device to an electronic output, such as an audio output, through a waterproof case are also provided.

Abstract: A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

Abstract: A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

Abstract: A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturing Candida sp. in a fermentation medium containing various defined substrates.

Abstract: The present invention provides modified promoters from Candida troplicalis CYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

Abstract: A multi-well cassette configuration and an instrument capable of accepting the cassette and thereafter pre-concentrating and purifying analytes from biological samples held in the cassette wells, such as human serum, for subsequent analysis by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS).

Abstract: The present invention provides antigenic peptides useful for the production of antibodies which selectively bind to an enzyme involved in the ?-oxidation of fatty acids and alkanes to ?,?-dicarboxylic acids in yeast. Antibodies which specifically bind to an enzyme involved in the ?-oxidation of fatty acids and alkanes to ?,?-dicarboxylic acids in yeast are also provided. In addition, methods of producing the subject antibodies, a method of detecting the presence and amount of a specific enzyme involved in the ?-oxidation of fatty acids and alkanes to ?,?-dicarboxylic acids, and a method of monitoring the degree of enzyme induction and/or enzyme stability in a mixture during ?-oxidation of fatty acids and alkanes to ?,?-dicarboxylic acids in yeast, are also provided.

Abstract: Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ?-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Abstract: Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ?-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Abstract: Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ?-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Abstract: The present invention provides methods of converting or increasing conversion of a fatty acid to its corresponding dicarboxylic acid. The methods comprise isolating a promoter from a yeast gene which gene is induced when the yeast is grown on a fatty acid or alkane substrate, and operably linking the promoter to a gene involved in dicarboxylic acid production to form an expression vector. Yeast cells are subsequently transformed with such an expression vector and cultured in a media containing an organic substrate biooxidizable to a mono- or polycarboxylic acid, and resultant yeast cells convert or increase conversion of fatty acids to their corresponding dicarboxylic acids. Examples of promoters that may be used in the methods of the present invention include those from C. tropicalis catalase, citrate synthase, 3-ketoacyl-CoA thiolase A, citrate synthase, O-acetylhomserine sulphydrylase, protease, carnitine O-acetyltransferase, hydratase-dehydrogenase, and epimerase genes.

Abstract: A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

Abstract: A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

Abstract: The present invention provides antigenic peptides useful for the production of antibodies which selectively bind to an enzyme involved in the &ohgr;-oxidation of fatty acids and alkanes to &agr;,&ohgr;-dicarboxylic acids in yeast. Antibodies which specifically bind to an enzyme involved in the &ohgr;-oxidation of fatty acids and alkanes to &agr;,&ohgr;-dicarboxylic acids in yeast are also provided. In addition, methods of producing the subject antibodies, a method of detecting the presence and amount of a specific enzyme involved in the &ohgr;-oxidation of fatty acids and alkanes to &agr;,&ohgr;-dicarboxylic acids, and a method of monitoring the degree of enzyme induction and/or enzyme stability in a mixture during &ohgr;-oxidation of fatty acids and alkanes to &agr;,&ohgr;-dicarboxylic acids in yeast, are also provided.