Abstract: The present invention is a method for assaying enzyme activity in a red blood sample. The method comprises these steps: (a) placing the following in a sample well: (1) a red blood sample containing an enzyme, (2) a substrate or substrates for the enzyme, (3) water, and (4) a buffer; (b) incubating the contents of the sample well for sufficient time and at sufficient temperature to allow for the formation of a fluorescent enzyme product should the enzyme be present in said red blood sample; (c) precipitating the hemoglobin; and (d) measuring the fluorescence of any fluorescent enzyme product formed in the sample well, directly from that sample well. The method of the invention may be used for assaying the activity of an enzyme, such as galactose-1-phosphate uridyl transferase (GALT) or biotinidase, in a red blood sample.

Abstract: The present invention is a method for assaying enzyme activity in a red blood sample. The method comprises these steps: (a) placing the following in a sample well: (1) a red blood sample containing an enzyme, (2) a substrate or substrates for the enzyme, (3) water, and (4) a buffer; (b) incubating the contents of the sample well for sufficient time and at sufficient temperature to allow for the formation of a fluorescent enzyme product should the enzyme be present in the red blood sample; (c) precipitating the hemoglobin; and (d) measuring the fluorescence of any fluorescent enzyme product formed in the sample well, directly from that sample well. The method of the invention may be used for assaying the activity of an enzyme, such as galactose-1-phosphate uridyl transferase (GALT) or biotinidase, in a red blood sample.

Abstract: The present invention is an absorbent paper piece having a plurality of samples zones adapted to receive a liquid sample. The paper comprises an absorbent paper piece having a plurality of sample zones, each sample zone having a perforation extending substantially around its perimeter, so as to substantially obstruct the capillary flow of the liquid sample from one of the sample zones having an there sample zone adjacent thereto, to the other sample zone. It is preferred that the absorbent paper piece be provided with barrier perforations interposed between adjacent sample zones, so as to provide a gap between the sample zones as an additional capillary flow barrier. The absorbent paper piece may be adapted to hold any desired sample in liquid form such as laboratory reagents, and biological samples, such as blood samples (here used to include blood, products or components of blood, and blood hemolysate).