Patents by Inventor Ronald D. Flannagan
Ronald D. Flannagan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 8188036Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: May 27, 2009Date of Patent: May 29, 2012Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
-
Patent number: 8071736Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.Type: GrantFiled: July 30, 2009Date of Patent: December 6, 2011Assignees: Pioneer Hi-Bred International, Inc., E.I. Dupont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
-
Patent number: 7993856Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.Type: GrantFiled: March 30, 2009Date of Patent: August 9, 2011Assignee: Pioneer Hi-Bred International, Inc.Inventors: Ronald D. Flannagan, Terry EuClaire Meyer
-
Patent number: 7910807Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.Type: GrantFiled: November 14, 2007Date of Patent: March 22, 2011Assignees: Pioneer Hi-Bred International, Inc., E.I. DuPont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
-
Patent number: 7803993Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.Type: GrantFiled: September 12, 2007Date of Patent: September 28, 2010Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Billy F. McCutchen, Cao Guo Yu
-
Patent number: 7790846Abstract: The invention provides Bacillus thuringiensis toxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated pesticidal proteins and pesticidal compositions. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: June 21, 2006Date of Patent: September 7, 2010Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Ronald D. Flannagan, André Abad
-
Patent number: 7696412Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: April 16, 2003Date of Patent: April 13, 2010Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
-
Publication number: 20090317891Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.Type: ApplicationFiled: July 30, 2009Publication date: December 24, 2009Applicants: Pioneer Hi-Bred International, Inc., E.I. du PONT de NEMOURS and COMPANYInventors: Andre Abad, Ronald D. Flannagan, Rafael Hermann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
-
Patent number: 7629449Abstract: In particular aspects of the invention, nucleic acid molecules encoding novel proteases identified in the midgut of Diabrotica virgfera virgfera (i.e., western corn rootworm) are provided. The nucleotide sequences encode novel cysteine proteases that belong to the cathepsin L-like subfamily of proteases. Variants and fragments of the nucleotide sequences that encode polypeptide sequences that possess proteolytic activity are further disclosed. Methods of using the nucleic acid sequences that encode novel proteases to protect a plant from an insect pest are provided. Particular embodiments also provide vectors, expression cassettes and transformed host cells comprising a protease nucleic acid of the invention.Type: GrantFiled: December 4, 2007Date of Patent: December 8, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
-
Patent number: 7629504Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding Cry9 ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.Type: GrantFiled: December 21, 2004Date of Patent: December 8, 2009Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Ronald D. Flannagan, Andre R. Abad
-
Publication number: 20090291896Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: ApplicationFiled: May 27, 2009Publication date: November 26, 2009Applicant: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
-
Patent number: 7605304Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: October 23, 2001Date of Patent: October 20, 2009Assignee: E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Nicholas B. Duck, Xiang Feng, Ronald D. Flannagan, Theodore W. Kahn, Lynne E. Sims
-
Publication number: 20090203042Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.Type: ApplicationFiled: March 30, 2009Publication date: August 13, 2009Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ronald D. Flannagan, John P. Mathis, Terry EuClaire Meyer
-
Patent number: 7572889Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.Type: GrantFiled: July 29, 2005Date of Patent: August 11, 2009Assignee: Pioneer Hi-Bred International, Inc.Inventors: Ronald D. Flannagan, Terry EuClaire Meyer
-
Patent number: 7557080Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.Type: GrantFiled: June 21, 2006Date of Patent: July 7, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: André Abad, Ronald D. Flannagan
-
Patent number: 7556936Abstract: Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.Type: GrantFiled: December 5, 2007Date of Patent: July 7, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Rafael Herrmann, Albert L. Lu, Billy F. McCutchen, James K. Presnail, Janet A. Rice, James F. Wong, Cao-Guo Yu
-
Patent number: 7541517Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding Cry9 ?-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes, and transformed plants comprising a nucleic acid of the invention.Type: GrantFiled: June 21, 2006Date of Patent: June 2, 2009Assignees: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Ronald D. Flannagan, André Abad
-
Publication number: 20090130672Abstract: Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.Type: ApplicationFiled: November 9, 2007Publication date: May 21, 2009Applicants: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Bin Hu, Cao Guo Yu
-
Patent number: 7534864Abstract: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins.Type: GrantFiled: May 8, 2006Date of Patent: May 19, 2009Assignee: Pioneer Hi-Bred International, Inc.Inventors: Ronald D. Flannagan, Terry EuClaire Meyer
-
Publication number: 20090075287Abstract: Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.Type: ApplicationFiled: September 11, 2008Publication date: March 19, 2009Applicants: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Andre R. Abad, Ronald D. Flannagan, Bin Hu, Billy F. McCutchen, Xiaofeng Sean Yang, Cao Guo Yu