Patents by Inventor Ronald G. Sosnowski
Ronald G. Sosnowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8114589Abstract: A method for electronically stabilizing hybridization of nucleic acids bound at a test site of a microelectronic device is described. First and second negatively charged nucleic acids are provided, the second nucleic acid being bound to the test site. A zwitterionic buffer having a conductance of less than 100 mS/cm is applied to the microelectronic device. A current is applied to the test site to positively bias the test site, such that the first negatively charged nucleic acid is transported to the positively biased test site having the bound the second negatively charged nucleic acid. At the test site, the first and second negatively charged nucleic acids hybridize. The zwitterionic buffer acquires a net positive charge under influence of the current, such that the positively charged zwitterionic buffer stabilizes the hybridization by reducing the repulsion between the first and second negatively charged nucleic acids.Type: GrantFiled: March 22, 2007Date of Patent: February 14, 2012Assignee: Gamida For Life B.V.Inventors: Ronald G. Sosnowski, William F. Butler, Eugene Tu, Michael I. Nerenberg, Michael J. Heller, Carl F. Edman
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Patent number: 7947486Abstract: A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.Type: GrantFiled: February 5, 2007Date of Patent: May 24, 2011Assignee: Gamida for Life B.V.Inventors: Michael J. Heller, Eugene Tu, Glen A. Evans, Ronald G. Sosnowski
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Publication number: 20100173792Abstract: A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.Type: ApplicationFiled: February 5, 2007Publication date: July 8, 2010Inventors: Michael J. Heller, Eugene Tu, Glen A. Evans, Ronald G. Sosnowski
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Publication number: 20100133118Abstract: In accordance with the present invention, there are provided systems for detecting hybridization of nucleic acids using electrochemical methods having improved sensitivity. Such systems include an electrode having a variably charged oligonucleotide probe and a redox probe. In some embodiments, the systems may further include a binding nexus having an immobilized reporter oligonucleotide probe, which hybridizes to a target nucleic acid sequence. The reporter oligonucleotide probe may be naturally charged, uncharged, or either partially negatively or positively charged. Further provided are methods for detecting the presence of a nucleic acid sequence of interest in a sample.Type: ApplicationFiled: November 23, 2009Publication date: June 3, 2010Applicant: ADNAVANCE TECHNOLOGIES, INC.Inventors: Ronald G. Sosnowski, Gabriel Baru Fassio, Zuxu Yao, Robert Haigis, Tao Ye
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Publication number: 20100114956Abstract: The present invention provides methods to infer STR allelic genotype from SNPs in a genome by obtaining statistical probabilities for the association of a plurality of SNPs in a genome with a Short Tandem Repeat (STR) locus allele for the genome to obtain a SNP constellation association value.Type: ApplicationFiled: October 13, 2009Publication date: May 6, 2010Applicant: Casework GeneticsInventors: Kevin McElfresh, Ronald G. Sosnowski
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Patent number: 7582421Abstract: This application includes methods for detecting single nucleotide polymorphisms (SNPs) in a sample using an electronically addressable microchip having a plurality of test sites. A sample nucleic acid is electronically biased, concentrated at, and immobilized to a test site on the microchip. A mixture comprising a first labeled probe and a second labeled probe is electronically hybridized to the sample nucleic acid to form first or second hybridized complexes. The first labeled probe is perfectly complementary to the first sample nucleic acid and the second labeled probe is complementary to the sample nucleic acid and contains a nucleotide that forms a mismatch with the nucleotide at the site of the polymorphism. The first or second hybridized complexes are detected by determining a signal intensity of the label of the first or second probe.Type: GrantFiled: September 16, 2002Date of Patent: September 1, 2009Assignee: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Michael I. Nerenberg, David M. Canter, Ray R. Radtkey, Ling Wang, James P. O'Connell
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Patent number: 7172864Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Type: GrantFiled: January 24, 2000Date of Patent: February 6, 2007Assignee: NanogenInventors: Michael J. Heller, Eugene Tu, Glen A. Evans, Ronald G. Sosnowski
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Patent number: 6753148Abstract: Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0).Type: GrantFiled: March 25, 2002Date of Patent: June 22, 2004Assignee: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Eugene Tu
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Publication number: 20040086917Abstract: Methods for electronic perturbation of fluorescence, chemilluminescence and other emissive materials provide for molecular biological analysis.Type: ApplicationFiled: July 18, 2003Publication date: May 6, 2004Applicant: Nanogen, Inc.Inventors: Michael J. Heller, Eugene Tu, Ronald G. Sosnowski, James P. O'Connell
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Publication number: 20030219804Abstract: Methods and compositions of matter are provided for the strand displacement amplification of target nucleic acids of interest using primer pair sets that are anchored to electronically addressable capture sites on a microarray. The primer pair sets may be individually bound to the capture sites or may comprise a unique branched primer pair moiety. The anchored primers allow for the simultaneous multiplex capture, amplification and detection of a target nucleic acid derived from any sample source.Type: ApplicationFiled: March 11, 2003Publication date: November 27, 2003Applicant: Nanogen, Inc.Inventors: Michael I. Nerenberg, Carl F. Edman, Lorelei P. Westin, Lana L. Feng, Geoffrey C. Landis, Ronald G. Sosnowski
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Publication number: 20030190632Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Type: ApplicationFiled: June 11, 2002Publication date: October 9, 2003Applicant: Nanogen, Inc.Inventors: Ronald G. Sosnowski, William F. Butler, Eugene Tu, Michael I. Nerenberg, Michael J. Heller, Carl F. Edman
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Publication number: 20030162214Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Type: ApplicationFiled: February 21, 2003Publication date: August 28, 2003Applicant: Nanogen, Inc.Inventors: Michael J. Heller, Eugene Tu, Glen A. Evans, Ronald G. Sosnowski
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Publication number: 20030073122Abstract: Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target.Type: ApplicationFiled: September 16, 2002Publication date: April 17, 2003Applicant: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Michael I. Nerenberg, David M. Canter, Ray R. Radtkey, Ling Wang, James P. O'Connell
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Patent number: 6531302Abstract: Methods and compositions of matter are provided for the strand displacement amplification of target nucleic acids of interest using primer pair sets that are anchored to electronically addressable capture sites on a microarray. The primer pair sets may be individually bound to the capture sites or may comprise a unique branched primer pair moiety. The anchored primers allow for the simultaneous multiplex capture, amplification and detection of a target nucleic acid derived from any sample source.Type: GrantFiled: April 12, 1999Date of Patent: March 11, 2003Assignee: Nanogen/Becton Dickinson PartnershipInventors: Michael I. Nerenberg, Carl F. Edman, Lorelei P. Westin, Lana L. Feng, Geoffrey C. Landis, Ronald G. Sosnowski
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Patent number: 6518022Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific microlocations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Type: GrantFiled: November 22, 1999Date of Patent: February 11, 2003Assignee: Nanogen, Inc.Inventors: Ronald G. Sosnowski, William F. Butler, Eugene Tu, Michael I. Nerenberg, Michael J. Heller, Carl F. Edman
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Patent number: 6468742Abstract: Methods are provided for the analysis and determination of the nature of single nucleic acid polymorphisms (SNPs) in a genetic target. In one method of this invention, the nature of the SNPs in the genetic target is determined by the steps of providing a plurality of hybridization complexes arrayed on a plurality of test sites on an electronically bioactive microchip, where the hybridization complex includes at least a nucleic acid target containing a SNP, a stabilizer probe having a sequence complementary to the target sequence and/or reporter probe, and a reporter probe having a selected sequence complementary to either the stabilizer or the same target sequence strand wherein a selected sequence of the reporter includes either a wild type nucleotide or a nucleotide corresponding to the SNP of the target.Type: GrantFiled: April 12, 1999Date of Patent: October 22, 2002Assignee: Nanogen, Inc.Inventors: Michael I. Nerenberg, David M. Canter, Ray R. Radtkey, Ling Wang, James P. O'connell, Ronald G. Sosnowski
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Publication number: 20020115098Abstract: Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0).Type: ApplicationFiled: March 25, 2002Publication date: August 22, 2002Applicant: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Eugene Tu
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Patent number: 6395493Abstract: Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0).Type: GrantFiled: August 24, 2000Date of Patent: May 28, 2002Assignee: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Eugene Tu
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Patent number: 6238624Abstract: A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.Type: GrantFiled: October 4, 1996Date of Patent: May 29, 2001Assignee: Nanogen, Inc.Inventors: Michael J. Heller, Eugene Tu, Glen A. Evans, Ronald G. Sosnowski
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Patent number: 6207373Abstract: Methods and apparatus are provided for the analysis and determination of the nature of repeat units in a genetic target. In one method of this invention, the nature of the repeat units in the genetic target is determined by the steps of providing a plurality of hybridization complex assays arrayed on a plurality of test sites, where the hybridization complex assay includes at least a nucleic acid target containing a simple repetitive DNA sequence, a capture probe having a first unique flanking sequence and n repeat units, where n=0,1,2 . . . , or fractions thereof, being complementary to the target sequence, and a reporter probe having a selected sequence complementary to the same target sequence strand wherein the selected sequence of the reporter includes a second unique flanking sequence and m repeat units, where m=0,1,2 . . . , or fractions thereof, but where the sum of repeat units in the capture probe plus reporter probe is greater than 0 (n+m>0).Type: GrantFiled: February 25, 1998Date of Patent: March 27, 2001Assignee: Nanogen, Inc.Inventors: Ronald G. Sosnowski, Eugene Tu