Patents by Inventor Ronald Godiska
Ronald Godiska has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9765343Abstract: Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.Type: GrantFiled: April 8, 2015Date of Patent: September 19, 2017Assignee: LUCIGEN CORPORATIONInventors: Ronald Godiska, David A. Mead, Nikolai V. Ravin
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Publication number: 20150218565Abstract: Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.Type: ApplicationFiled: April 8, 2015Publication date: August 6, 2015Applicant: Lucigen CorporationInventors: Ronald Godiska, David A. Mead, Nikolai V. Ravin
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Patent number: 9029134Abstract: Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.Type: GrantFiled: January 12, 2007Date of Patent: May 12, 2015Assignee: Lucigen CorporationInventors: Ronald Godiska, David A. Mead, Nikolai V. Ravin
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Patent number: 8623652Abstract: A system for ligase-free cloning and/or expressing a target gene is described herein. A preferred version of the invention includes an E. coli host. The host preferably includes a T7 RNA polymerase gene comprising a T7gpl coding sequence, a lacUV5 promoter, and a lac operator. The host preferably further includes a lacI gene comprising a lacI coding sequence with an ATG start codon, a promoter derived from the lacql allele, and a translational enhancer derived from a 5? RNA leader sequence of T7 gene 10. The invention further includes a low-copy plasmid vector comprising a T7 promoter a lac operator operationally linked to the T7 promoter. The system is configured to inhibit target gene expression when uninduced and to permit gene expression upon induction by auto-induction.Type: GrantFiled: April 6, 2010Date of Patent: January 7, 2014Assignee: Lucigen CorporationInventors: Eric Steinmetz, Ronald Godiska, David A. Mead
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Publication number: 20100255561Abstract: A system for ligase-free cloning and/or expressing a target gene is described herein. A preferred version of the invention includes an E. coli host. The host preferably includes a T7 RNA polymerase gene comprising a T7gpl coding sequence, a lacUV5 promoter, and a lac operator. The host preferably further includes a lacI gene comprising a lacI coding sequence with an ATG start codon, a promoter derived from the lacql allele, and a translational enhancer derived from a 5? RNA leader sequence of T7 gene 10. The invention further includes a low-copy plasmid vector comprising a T7 promoter a lac operator operationally linked to the T7 promoter. The system is configured to inhibit target gene expression when uninduced and to permit gene expression upon induction by auto-induction.Type: ApplicationFiled: April 6, 2010Publication date: October 7, 2010Inventors: Eric Steinmetz, Ronald Godiska, David A. Mead
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Patent number: 7723103Abstract: Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3? termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.Type: GrantFiled: January 8, 2007Date of Patent: May 25, 2010Assignee: Lucigen CorporationInventors: David A. Mead, Ronald Godiska, Thomas W. Schoenfeld, Spencer Hermanson
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Publication number: 20090263873Abstract: Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.Type: ApplicationFiled: January 12, 2007Publication date: October 22, 2009Applicant: LUCIGEN CORPORATIONInventors: Ronald Godiska, David A. Mead, Nikolai V. Ravin
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Publication number: 20080166773Abstract: Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3? termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.Type: ApplicationFiled: January 8, 2007Publication date: July 10, 2008Applicant: LUCIGEN CORPORATIONInventors: David A. Mead, Ronald Godiska, Thomas W. Schoenfeld, Spencer Hermanson
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Patent number: 7018627Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel macrophage-derived C—C chemokine designated “Macrophage Derived Chemokine” (MDC), and polypeptide fragments and analogs thereof. Also provided are materials and methods for the recombinant or synthetic production of the chemokine, fragments, and analogs; and purified and isolated chemokine protein, and polypeptide fragments and analogs thereof. Also provided are antibodies reactive with the chemokine and methods of making and using all of the foregoing. Also provided are assays for identifying modulators of MDC chemokine activity.Type: GrantFiled: September 28, 1998Date of Patent: March 28, 2006Assignee: Icos CorporationInventors: Patrick W. Gray, David H. Chantry, Michael C. Deeley, Carol J. Raport, Ronald Godiska
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Publication number: 20060057112Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel macrophage-derived C-C chemokine designated “Macrophage Derived Chemokine” (MDC), and polypeptide fragments and analogs thereof. Also provided are materials and methods for the recombinant or synthetic production of the chemokine, fragments, and analogs; and purified and isolated chemokine protein, and polypeptide fragments and analogs thereof. Also provided are antibodies reactive with the chemokine and methods of making and using all of the foregoing. Also provided are assays for identifying modulators of MDC chemokine activity.Type: ApplicationFiled: October 28, 2005Publication date: March 16, 2006Applicant: ICOS CORPORATIONInventors: Patrick Gray, David Chantry, Michael Deeley, Carol Raport, Ronald Godiska
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Publication number: 20040244065Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.Type: ApplicationFiled: February 20, 2004Publication date: December 2, 2004Inventors: David Alan Mead, Ronald Godiska
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Publication number: 20040229359Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.Type: ApplicationFiled: December 19, 2003Publication date: November 18, 2004Inventors: David Alan Mead, Ronald Godiska
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Patent number: 6790947Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C—C chemokine designated MDC. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein.Type: GrantFiled: June 7, 1995Date of Patent: September 14, 2004Assignee: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray
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Patent number: 6709861Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.Type: GrantFiled: November 15, 2001Date of Patent: March 23, 2004Assignee: Lucigen Corp.Inventors: David Alan Mead, Ronald Godiska
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Publication number: 20040038401Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.Type: ApplicationFiled: November 15, 2001Publication date: February 26, 2004Applicant: Lucigen CorporationInventors: David Alan Mead, Ronald Godiska
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Patent number: 6498015Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C-C chemokine designated Macrophage Derived Chemokine (MDC), and polypeptide analogs thereof. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein, and polypeptide analogs thereof. Also provided are antibodies reactive with the chemokine and methods of making and using all of the foregoing.Type: GrantFiled: September 26, 1997Date of Patent: December 24, 2002Assignee: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray, Carol J. Raport
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Publication number: 20020102642Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C—C chemokine designated MCP-5. Also provided are purified and isolated chemokine protein, fragments and polypeptide analogs thereof, antibodies thereto, and materials and methods for the recombinant production thereof. These products are useful in therapeutic, diagnostic and medical imaging applications.Type: ApplicationFiled: June 1, 2001Publication date: August 1, 2002Applicant: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray
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Patent number: 6348574Abstract: DNA sequences encoding seven novel seven transmembrane receptors and variants thereof are disclosed as well as materials and methods for production of the same by recombinant techniques. Antibody substances specific for each of the seven transmembrane receptors are disclosed as useful for the modulation of the ligand/receptor binding reactions of the receptors.Type: GrantFiled: June 1, 1998Date of Patent: February 19, 2002Assignee: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray, Vicki Louise Schweickart
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Patent number: 6320023Abstract: The present invention provides purified and isolated polynucleotide sequences encoding a novel human macrophage-derived C-C chemokine designated MDC. Also provided are materials and methods for the recombinant production of the chemokine, and purified and isolated chemokine protein.Type: GrantFiled: June 7, 1995Date of Patent: November 20, 2001Assignee: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray
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Patent number: 6107475Abstract: DNA sequences encoding seven novel seven transmembrane receptors and variants thereof are disclosed as well as materials and methods for production of the same by recombinant techniques. Antibody substances specific for each of the seven transmembrane receptors are disclosed as useful for the modulation of the ligand/receptor binding reactions of the receptors.Type: GrantFiled: April 26, 1999Date of Patent: August 22, 2000Assignee: ICOS CorporationInventors: Ronald Godiska, Patrick W. Gray, Vicki Louise Schweickart